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A kit for detecting jwa expression in cancer or malignant tumor tissue

A kind of malignant tumor, kit technology, applied in the field of biological and medical detection

Inactive Publication Date: 2011-12-28
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In fact, even though different patients with similar clinicopathological features may have completely different responses and effects to the same treatment plan, some of which may not be suitable for one or some patients at all, but so far there is no particularly reliable method to judge Whether a malignant tumor patient needs to receive a certain treatment, that is, individualized treatment

Method used

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  • A kit for detecting jwa expression in cancer or malignant tumor tissue
  • A kit for detecting jwa expression in cancer or malignant tumor tissue
  • A kit for detecting jwa expression in cancer or malignant tumor tissue

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Example 1 Immunohistochemical detection of the expression level of JWA in gastric cancer tissue (taking paraffin section of gastric cancer tissue as an example)

[0031] 1. Preparation of cancer tissue sections before staining

[0032] A variety of commercial or entrusted biotechnology companies or the laboratory prepared by itself containing gastric cancer

[0033]Paraffin sections of tumor tissues (4 μm in thickness) were dewaxed with xylene for 3 times, 5 min each time, rinsed with 100%, 100%, 95%, 90%, 80%, and 70% ethanol aqueous solution, 3 min each time, Then rinse with distilled water for 5 minutes. Then rinse with PBS (phosphate buffered saline, pH=7.4) 3 times, 3 min each time.

[0034] Antigen retrieval method (1) Place slices in 10mM citrate solution (pH6.0), heat in a microwave oven, keep the temperature at 92-100°C, and last for 2×5min; after the first 5min, the visible Whether the citrate solution has decreased evaporation, if there is a decrease in ev...

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Abstract

The invention relates to the field of biological and medical detection, in particular to a kit for detecting JWA expression in cancer or malignant tumor tissue. A kit for detecting JWA expression in cancer or malignant tumor tissue, characterized in that the kit includes primary antibody, general secondary antibody-polymer solution, chromogen, and blocking solution. The invention provides a kit for detecting the expression level of JWA protein in tumor tissue, and the result can be used as an important basis for judging the prognosis of tumor patients and formulating an individualized treatment plan, that is, detecting the expression level of JWA in tumor tissue, and according to the expression level of JWA levels to help determine treatment options. Obviously, the content of the present invention has very important original significance and application value. The expression level of JWA in cancer tissue can be used as an important molecular marker to measure the degree of tumor malignancy, formulate chemotherapy regimens, and judge prognosis.

Description

technical field [0001] The invention relates to the field of biological and medical detection, in particular to a kit for detecting JWA expression in cancer or malignant tumor tissue. Background technique [0002] Malignant tumor is a disease that seriously endangers human health, and has become the first place in the classification of causes of death. With the development of modern biomedical technology, people's understanding of the law of the occurrence and development of malignant tumors has deepened, and a series of genes related to malignant tumors have been discovered. With the deepening of research, the functions of these genes have been gradually clarified. Therefore, genetic diagnosis New malignant tumor treatment strategies with gene therapy as the core have also emerged as the times require. [0003] JWA, namely the ARL6IP5 gene, is a new gene that is induced by retinoic acid to regulate cell differentiation and is first cloned from a cDNA library constructed by...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/574G01N33/558
Inventor 周建伟陈颜夙张建兵李爱萍谈永飞强福林
Owner NANJING MEDICAL UNIV
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