High-quality exosome and preparation method thereof

An exosome and bold technology, applied in the fields of biology and medicine, can solve the problems of large volume, difficult to meet clinical needs, low yield, etc.
CN104894067AActive Publication Date: 2015-09-09ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2015-09-09

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Abstract

The present invention discloses a high-quality exosome and a preparation method thereof, and particularly relates to an exosome, which is vesicles produced by cells and extracellular secreted, wherein the vesicles have the following characteristics that: a) the diameter is 30-90 nm; b) the density is 1.11-1.19 g / ml; c) and the vesicles contain one or a plurality of feature molecules such as TfR, LAMP, HSP70, HSC70 and CD9. The exosome of the present invention has characteristics of good uniformity and high safety, and can be subjected to mass production through the method of the present invention so as to meet clinical requirements.
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Description

technical field

[0001] The invention relates to the fields of biology and medicine, in particular to an exosome with good uniformity and high safety and a method for preparing the exosome on a large scale. Background technique

[0002] Malignant tumors are major diseases that seriously threaten human life and quality of life. Tumor immunotherapy is a necessary supplement to the three major treatment methods of surgery, chemotherapy and radiotherapy, and it is also a hot spot in tumor treatment and immunology research. With the successive discovery and identification of many tumor-specific antigens and tumor-associated antigens, as well as the deepening of people's research on the mechanism of antigen presentation and T cell activation, the application of various forms of tumor vaccines to induce the body's T cell-mediated The application research of specific anti-tumor immune function has been developed rapidly. However, due to the weak immunogenicity of most tumors, in th...

Claims

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