Method for preparing water/oil/water (W/O/W) animal vaccine oil emulsion adjuvant

Abstract

The invention discloses a method for preparing a water / oil / water (W / O / W) animal vaccine oil emulsion adjuvant, and relates to the field of the preparation of animal vaccine oil emulsion adjuvants. The method comprises the following steps of: (1) preparing a substance A, namely adding a nonionic segmented copolymer L180 into a NaCl solution, dissolving and mixing uniformly; (2) preparing a substance B, namely adding a chimeric copolymer P1003 and a hydrophilic surfactant into the NaCl solution, dissolving and mixing uniformly; (3) preparing a substance C, namely adding the substance A and the surfactant into light mineral oil, emulsifying and homogenizing; (4) preparing a substance D, namely adding the substance B into the substance C, and mixing uniformly; and (5) homogenizing the substance D at high pressure to obtain the W / O / W animal vaccine oil emulsion adjuvant. The adjuvant contains a uniform submicron oil carrier and has high stability, and the adjuvant can not be demixed within 12 months at the temperature of 4 DEG C, can not be demixed within 6 months at room temperature and can not be demixed within 10 days at the temperature of 40 DEG C.

Description

technical field [0001] The invention relates to the field of preparation of animal vaccine oil emulsion adjuvant, in particular to a preparation method of W / O / W type animal vaccine oil emulsion adjuvant. Background technique [0002] Vaccine adjuvants can improve the immune effect of vaccines. Its main role in immunity is to enhance the immune response of the body's immune system to antigens or immunogens, enhance the intensity of immune responses and the durability of responses, thereby improving the body's resistance to viruses or bacteria. resistance. [0003] The research on adjuvants is developed to adapt to the application of vaccines. With the development of breeding industry and breeding technology, the amount of animal vaccines is increasing, and the amount of adjuvants is also increasing. According to statistics, there are currently 70 domestic animal vaccine manufacturers that have passed the veterinary drug GMP certification, and the total vaccine capacity in th...

AI Technical Summary

Problems solved by technology

Oil-emulsion adjuvants can improve the immune effect of vaccines, but the type and amount of oil and emulsifier are closely related to the safety of the vaccine. Usually, the side effects of animals are more serious after inoculation with such vaccines

[0007] Currently commonly used oil-emulsion adjuvants have a tendency to reside at the injection site, and are prone to aseptic abscesses and granulomas near the inoculation site, and may cause severe pain during inoculation, and are prone to delamination and poor stability. It can only ensure that there will be no delamination within 2 months of storage at 4°C, and the viscosity is large, the performance of the bioactive agent used is poor, and the side effects on animals are large. The W / O / W type oil emulsion adjuvant of the present invention can make up for the above deficiencies