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Method for culturing lactic acid bacteria

A culture method and technology of lactic acid bacteria, applied in the field of microorganisms, can solve problems such as low efficiency, slow production of lactic acid bacteria, and low quality of silage

Inactive Publication Date: 2012-02-01
SHANGHAI HAIDI GARDENING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to overcome the technical problems of slow production of lactic acid bacteria in the lactic acid bacteria culture method in the prior art, low efficiency, and low quality of silage prepared by utilizing it

Method used

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  • Method for culturing lactic acid bacteria

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Experimental program
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Effect test

Embodiment

[0013] 1. Use the coating method to inoculate the bacteria on the MRS medium

[0014] (1) Take 25g of fresh corn plant, put it into a triangular flask filled with 225ml sterilized saline, add a small amount of glass beads and shake it on a shaker for 30min;

[0015] (2) Use a sterilized straw to draw 1ml of the above liquid, and slowly inject it into a test tube containing 9ml sterilized normal saline;

[0016] (3) Take another sterilized straw, and dilute the liquid in increments of 10 times according to the above operation sequence, so that every time the increment is increased, replace it with a sterilized straw;

[0017] (4) From the bacterium colonies cultivated by the above method, catch a colony of Pediococcus pentosaceus (Pediococcus pentosaceus), Lactobacillus plantarum (Lactobacillus plantarum), and Lactobacillus casei (Lactobacillus casei) respectively with a fishing ring, and then respectively Doubling diluted to 10 -6 、10 -5 、10 -4 、10 -3 ;

[0018] (5) Use ...

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Abstract

The invention relates to a method for culturing lactic acid bacteria and belongs to the technical field of microbes. The method comprises the following steps of: inoculating strains to a de Man, Rogosa and Sharp (MRS) medium by adopting a coating method, and performing anaerobic culture. The lactic acid bacteria cultured by the method have more colonies and larger individuals, and can be used for preparing high-quality silage.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for cultivating lactic acid bacteria. Background technique [0002] Lactic acid bacteria refers to the general term for a class of non-spore-forming, Gram-positive bacteria whose main product is lactic acid. Bacteria that can produce lactic acid from the fermentation of glucose or lactose are collectively called lactic acid bacteria. Silage is a kind of roughage obtained by chopping green feed with a moisture content of 65% to 75%, and inhibiting the reproduction of various bacteria through the fermentation of anaerobic lactic acid bacteria under airtight and hypoxic conditions. Silage has a sour smell, is soft and juicy, has good palatability, is rich in nutrition, and is good for long-term storage. It is an excellent feed source for livestock. Lactic acid bacteria are the dominant microorganisms in the silage fermentation process. Lactic acid bacte...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/245C12R1/25C12R1/01
Inventor 郭景鹏沙飞翔丁国才
Owner SHANGHAI HAIDI GARDENING