Method for Tephritidae species identification and special PCR anterior system therefor

Abstract

The invention discloses a method for Tephritidae species identification and a special PCR (polymerase chain reaction) anterior system therefore. The method provided in the invention comprises the steps of: (1) adding the genome DNA of a Tephritidae to be tested into a PCR anterior system for PCR amplification, thus obtaining a PCR amplification product; (2) sequencing the PCR amplification product, and identifying the species of the tested Tephritidae through comparison with existing sequences. The PCR anterior system is composed of water and solutes with concentrations as the following: 12% of 10*Taq buffer by volume, 1.5nM of MgCl2, 0.1mM of dNTP, 0.0006mM of an upstream primer shown in sequence 1, 0.0006mM of a downstream primer shown in sequence 2, and 0.02U / microliter of a TaqDNA polymerase. The method provided in the invention has the advantages of: (1) realization of species identification to Tephritidae samples in different life phases and with poor integrity as well as different geographical origins; (2) maximum maintenance of the form integrity of tested samples; (3) strong versatility of a standardized operation and technology.

Description

Technical field [0001] The invention relates to a method for identification of fruit fly species and its special pre-PCR system. Background technique [0002] Fruit flies belong to the Diptera family Tephritidae and are widely distributed throughout the world, mostly in tropical and subtropical regions, with about 500 genera and 4500 species. More than 150 species of fruit flies have serious economic hazards. They spread by humans at long distances with their eggs, larvae, or pupae along with host fruits, packaging, and transportation, harming economic plants such as fruit trees and vegetables. They are highly regarded by the phytosanitary and agricultural departments of various countries. s concern. [0003] The species identification of fruit flies is mainly based on the morphological characteristics of adults. Although it is fast, simple and low-cost, it also has some limitations. Especially in distinguishing the similar species of fruit flies, the specimens are required to ha...

AI Technical Summary

Problems solved by technology

In addition, it is very difficult to identify the species of fruit fly larvae, and in most cases it is impossible to achieve, but most of the eggs and larvae intercepted at the ports in our country are the eggs and larvae of fruit flies, and it takes a long time to identify the species after raising the larvae to adults. Directly affect the operation speed and quality of quarantine at the port

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