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Tidal bioreactor for animal cell culture

A technology of animal cell culture and bioreactor, which is applied in the direction of bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, tissue cell/virus culture device, etc. , High production and operation costs, low cell yield and other issues, to achieve the effect of small occupied space, small equipment investment and high yield

Active Publication Date: 2012-03-21
向开兴
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For the deficiencies of the existing technology: such as the large space occupied by the rotary bottle machine, high input costs, high production and operation costs, low cell yield, limited to the lack of cell type cultivation, etc.

Method used

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  • Tidal bioreactor for animal cell culture
  • Tidal bioreactor for animal cell culture
  • Tidal bioreactor for animal cell culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A tidal type animal cell culture bioreactor, which also includes a culture medium liquid tank (3) and a three-air jacketed constant temperature incubator (18); wherein, the upper end of the medium liquid tank (3) is fixed with a liquid Tank gas filter (4), PH value detection electrode (5), sugar detection electrode (6), dissolved oxygen detection electrode (7), suction circulation pipe a (10), liquid inlet circulation pipe a (11) and replenishment The liquid inlet pipe (9); the three-gas clamp water jacket constant temperature incubator (18) is equipped with a cell culture reaction tank (17) filled with cell culture carriers (25), and the three gas clamp water jacket constant temperature incubator (18) One side is provided with the I peristaltic pump (14), the II peristaltic pump (15) and the III peristaltic pump (16), and the cell culture reaction tank (17) is provided with stirring device (20), gas filter (22) , liquid level electrode (21), liquid inlet circulation pi...

Embodiment 2

[0041] A kind of tidal type animal cell culture bioreactor of the present invention, such as figure 1 As shown, it also includes a work frame (1); a heating constant temperature water tank (2) and a culture medium replenishment or recovery bottle (30) are placed on the lower floor of the work frame (1), and the three-air clamp water jacket constant temperature incubator (18) is placed On the upper layer of the work frame (1), each component is connected according to the connection relationship described in Embodiment 1.

Embodiment 3

[0043] A kind of tidal type animal cell culture bioreactor of the present invention, reactor such as Image 6 Shown, wherein the water interlayer outside the culture medium holding liquid tank (3) is communicated with heating constant temperature water tank (2) by hot water circulating water pump (37) and circulating water pipe (43); (as Figure 7 Shown), the culture medium tank (3) is placed next to the work frame, and other components are the same as in embodiment 1, and are connected according to the connection relationship described in embodiment 1.

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Abstract

The invention provides a tidal bioreactor for animal cell culture. The tidal bioreactor for the animal cell culture mainly comprises a culture medium accommodating tank (3) and a three-gas water jacket constant-temperature culture box (18), wherein a cell culture reaction tank (17) is arranged inside the three-gas water jacket constant-temperature culture box (18); the culture medium accommodating tank (3) is arranged inside a heating constant-temperature water tank (2), or a water interlayer outside the culture medium accommodating tank (3) is communicated with the heating constant-temperature water tank (2) through a hot water circulating pump (37); the cell culture reaction tank (17) is communicated with the culture medium accommodating tank (3) through a third peristaltic pump (16); the culture medium accommodating tank (3) is communicated with a culture medium supplementing or recycling bottle (30) through a second peristaltic pump (15); and the culture medium supplementing or recycling bottle (30) is communicated with the culture medium accommodating tank (3) through a first peristaltic pump (14). The tidal bioreactor for the animal cell culture is small in investment, smallin occupied space, low in production cost and running cost, and high in yield.

Description

technical field [0001] The invention belongs to the field of biological cell culture reactors, and is suitable for food industry processing and production, agricultural and forestry biopesticides, biological products, biopharmaceuticals, biological vaccines, high-density large-scale cell production and cultivation, and subculture of biomedical clone cells, specifically a A tidal animal cell culture bioreactor. Background technique [0002] In the prior art, most of the cell cultures in agricultural and forestry biopesticides, biological products, biopharmaceuticals, and biovaccine pharmaceutical factories are cultivated in incubators with test bottles, and they are limited to experimental types and production types. . The spinner machine occupies a wide area, has high input costs, high production and operation costs, low cell yield, and is limited to the cultivation of cell types. Contents of the invention [0003] In view of the deficiencies of the existing technology: ...

Claims

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Application Information

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IPC IPC(8): C12M3/04C12M1/36C12M1/34C12M1/02
CPCC12M41/14C12M41/22
Inventor 向开兴陶伯辉
Owner 向开兴