Industrialized production method of Aspergillus oryzae strain for fermentation
A production method and koji enzyme technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as few large-scale production of strains, and achieve the effects of increasing yield and reducing costs.
Inactive Publication Date: 2012-03-21
湖北土老憨生态农业科技股份有限公司
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Problems solved by technology
At present, there are many reports on strain mutagenesis, strain screening and synergistic fermentation of different types of strains, but few reports on large-scale production of strains. The present invention solves the problem of large-scale production of strains from the aspect of expanded cultivation of Aspergillus oryzae kind of technical problem
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[0025] 1) Activate the strain of Aspergillus oryzae 3.042 from Huyao, and inoculate the strain of Aspergillus oryzae 3.042 from Huyao on the slant of the bean juice medium test tube under sterile conditions. The preparation method of the bean juice medium is as follows: : Soybean juice: 1000mL (4-5°Be), soluble starch 20g, MgSO 4 .7H 2 O 0.5g, (NH 4 ) 2 SO 4 0.5g, 20-25g agar powder, pH value 6.5-7.0, sterilized at 0.1Mpa for 30 minutes, the preparation process of soybean juice is to select 100g of fresh soybeans, soak them in water and remove them, then add 5-6 times the amount of soybeans in water , boil for 2-5 hours, pay attention to replenishing water when cooking beans, so that 100mL soybean juice is finally obtained by filtering with absorbent cotton, and this soybean juice is 5 ° Be soybean juice. During the cultivation process, the cultivation temperature was controlled at 30°C, and the cultivation time was 4 days, until the yellow-green spores covered the slope....
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Abstract
An industrialized production method of Aspergillus oryzae strain for fermentation. Aspergillus oryzae Shanghai brewing 3.042 strains are activated, treated with erlenmeyer flask enlargement culture and cultured in a culture room, namely the erlenmeyer flask strains are treated with further enlargement culture in the culture room. A medium formula is as below: 100 jin of a mixture of wheat bran and soybean meal in a ratio of 4:6 is added with 48-52 jin of water; The medium material is steamed in a charging steam basket for 30 min, cooled to 35-40 DEG C and inoculated with an inoculation proportion of 0.3%. The inoculated yeast material is placed in a yeast fan in a culture room for culture, and the yeast material has a thickness of 2.5-3 cm, and a culture time is 7d. The method of the invention can be utilized to produce Aspergillus oryzae strain with high enzyme production vitality and is capable of fermenting soybean paste, fermented soya bean and soy rapidly.
Description
technical field [0001] The invention belongs to a production method of aspergillus oryzae strains for fermentation, and in particular relates to a method for large-scale production of aspergillus oryzae strains. Background technique [0002] Fermentation process includes traditional fermentation process and artificial inoculation fermentation process. Since the traditional fermentation process has the characteristics of long growth cycle, difficult management, and unsafe product hygiene, it is increasingly unable to keep up with the needs of modern production. In order to improve production efficiency and ensure Market supply, the artificial inoculation fermentation process is generally adopted at present. Huyao 3.042 Aspergillus oryzae has the characteristics of fast growth, easy management and high enzyme production activity, and is often used in the inoculation fermentation of traditional soybean paste, tempeh and soy sauce. With the expansion of industrial production sc...
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IPC IPC(8): C12N1/14C12R1/69
Inventor 陈世贵付彩霞杨世贵
Owner 湖北土老憨生态农业科技股份有限公司