Food waste fermentation composite bacteria and preparation method thereof

A technology for fermenting composite bacteria and kitchen waste, applied in biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve problems such as heavy odor in garbage dumps, polluted land, air and water sources, and human hazards, and achieve Strong fat decomposition function, prevention and control of soil-borne diseases, strong amylase activity

Active Publication Date: 2012-03-28
SHANDONG SUKAHAN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, food waste is difficult to ferment, and there is no large-scale use of food waste treatment products in China
[0003] At present, the treatment of food waste mainly includes feeding livestock, oil extraction, incineration, landfill, etc., but the above treatment methods have the following hazards: 1. Food waste is directly fed to chickens, pigs and other livestock, It is easy to cause animal diseases, and the formation of a biological chain will eventually affect human health. Now the country has banned food waste from feeding livestock; 2. The oil extracted from food waste, that is, waste oil, is very likely to Flowing to the dining table will eventually affect human healt

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] High-density culture of Trichoderma viride, Bacillus subtilis, Rhizopus niger, photosynthetic bacteria, Saccharomyces cerevisiae and Bacillus licheniformis:

[0044] (1) Trichoderma viride: Inoculate the Trichoderma viride strain on the purified slant medium into a shake flask filled with an activated medium with a volume ratio of 15%, and the activated medium is wort potato sucrose medium , at a culture temperature of 30°C and a shaker flask rotation speed of 180rpm, activate and cultivate for 32 hours; inoculate the Trichoderma viride strains that have been activated and cultured into a fermenter with a volume ratio of 50% fermentation medium, and the inoculum volume is 1% by volume , the formula of the fermentation medium is glucose 8g / l, corncob 5g / l, calcium chloride 0.2g / l, magnesium sulfate 0.1g / l, potassium dihydrogen phosphate 18g / l, yeast extract 8g / l, sulfuric acid Ferrous 3mg / l, zinc sulfate 1.0mg / l, cobalt chloride 3.5mg / l and manganese sulfate 1.2mg / l, at ...

Embodiment 2

[0052] High-density culture of Trichoderma viride, Bacillus subtilis, Rhizopus niger, photosynthetic bacteria, Saccharomyces cerevisiae and Bacillus licheniformis:

[0053] (1) Trichoderma viride: Inoculate the Trichoderma viride strain on the purified slant culture medium into a shaker flask filled with a 30% volume ratio of activated medium, which is wort potato sucrose medium , at a culture temperature of 30°C and a shaker flask rotation speed of 140rpm, activate and cultivate for 48 hours; inoculate the Trichoderma viride strains that have been activated and cultured into a fermenter with a volume ratio of 70% fermentation medium, and the inoculum volume is 3% by volume , the formula of the fermentation medium is glucose 12g / l, corn cob 6g / l, calcium chloride 0.6g / l, magnesium sulfate 0.5g / l, potassium dihydrogen phosphate 22g / l, yeast extract 12g / l, sulfuric acid Ferrous 7mg / l, zinc sulfate 1.8mg / l, cobalt chloride 4.0mg / l and manganese sulfate 1.8mg / l, at a culture tempe...

Embodiment 3

[0061] High-density culture of Trichoderma viride, Bacillus subtilis, Rhizopus niger, photosynthetic bacteria, Saccharomyces cerevisiae and Bacillus licheniformis:

[0062] (1) Trichoderma viride: inoculate the Trichoderma viride strain on the purified slant medium into a shaker flask filled with an activated medium with a volume ratio of 20%, and the activated medium is wort potato sucrose medium , at a culture temperature of 30°C and a shaker flask rotation speed of 160rpm, activate and cultivate for 36 hours; inoculate the Trichoderma viride strains that have been activated and cultured into a fermenter with a volume ratio of 60% fermentation medium, and the inoculum volume is 2% by volume , the formula of the fermentation medium is glucose 10g / l, corn cob 5.5g / l, calcium chloride 0.4g / l, magnesium sulfate 0.3g / l, potassium dihydrogen phosphate 20g / l, yeast extract 10g / l, Ferrous sulfate 5mg / l, zinc sulfate 1.5mg / l, cobalt chloride 3.7mg / l and manganese sulfate 1.6mg / l, at ...

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Abstract

The invention discloses food waste fermentation composite bacteria and a preparation method thereof, and the preparation method comprises the following steps: respectively performing high-density culture on strains of trichoderma viride, bacillus subtilis, bread mold, photosynthetic bacteria, saccharomyces cerevisiae and bacillus licheniformis, performing dehydration and drying on various types of monoxenie obtained by high-density culture to prepare hypopus microbial dry powder, and then mixing according to the weight ratio of 20-45 parts of the trichoderma viride, 10-15 parts of the bacillus subtilis, 20-30 parts of the bread mold, 5-10 parts of photosynthetic bacteria, 7-15 parts of saccharomyces cerevisiae and 10-15 parts of bacillus licheniformis to get the food waste fermentation composite bacteria. The food waste fermentation composite bacteria are good in fermentation effect for treating food waste and significant in deodorization effect, and the food waste after using the composite bacteria for fermentation treatment can be used as an organic fertilizer, thereby changing the waste into valuable, avoiding polluting a water source, the land and the air and avoiding harming human bodies.

Description

technical field [0001] The invention relates to the technical field of bioengineering microbial fermentation, in particular to a composite food waste fermentation bacterium and a preparation method thereof. Background technique [0002] Compared with ordinary garbage, food waste has the following characteristics: 1. Food waste has high oil content and high viscosity; 2. Food waste has high salt content, and microorganisms are not easy to survive; 3. Food waste is piled up densely and is not easy to ventilate; 4. , High moisture content; 5, High organic matter content. Therefore, food waste is difficult to ferment, and there is no successful large-scale use of food waste treatment products in China. [0003] At present, the treatment of food waste mainly includes feeding livestock, oil extraction, incineration, landfill, etc., but the above treatment methods have the following hazards: 1. Food waste is directly fed to chickens, pigs and other livestock, It is easy to cause ...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/14C12N1/18C12N1/20B09B3/00C12R1/01C12R1/10C12R1/125C12R1/845C12R1/865C12R1/885
Inventor 韩威华于伟陈永科
Owner SHANDONG SUKAHAN BIO TECH
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