Food waste fermentation composite bacteria and preparation method thereof
A technology for fermenting composite bacteria and kitchen waste, applied in biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve problems such as heavy odor in garbage dumps, polluted land, air and water sources, and human hazards, and achieve Strong fat decomposition function, prevention and control of soil-borne diseases, strong amylase activity
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Embodiment 1
[0043] High-density culture of Trichoderma viride, Bacillus subtilis, Rhizopus niger, photosynthetic bacteria, Saccharomyces cerevisiae and Bacillus licheniformis:
[0044] (1) Trichoderma viride: Inoculate the Trichoderma viride strain on the purified slant medium into a shake flask filled with an activated medium with a volume ratio of 15%, and the activated medium is wort potato sucrose medium , at a culture temperature of 30°C and a shaker flask rotation speed of 180rpm, activate and cultivate for 32 hours; inoculate the Trichoderma viride strains that have been activated and cultured into a fermenter with a volume ratio of 50% fermentation medium, and the inoculum volume is 1% by volume , the formula of the fermentation medium is glucose 8g / l, corncob 5g / l, calcium chloride 0.2g / l, magnesium sulfate 0.1g / l, potassium dihydrogen phosphate 18g / l, yeast extract 8g / l, sulfuric acid Ferrous 3mg / l, zinc sulfate 1.0mg / l, cobalt chloride 3.5mg / l and manganese sulfate 1.2mg / l, at ...
Embodiment 2
[0052] High-density culture of Trichoderma viride, Bacillus subtilis, Rhizopus niger, photosynthetic bacteria, Saccharomyces cerevisiae and Bacillus licheniformis:
[0053] (1) Trichoderma viride: Inoculate the Trichoderma viride strain on the purified slant culture medium into a shaker flask filled with a 30% volume ratio of activated medium, which is wort potato sucrose medium , at a culture temperature of 30°C and a shaker flask rotation speed of 140rpm, activate and cultivate for 48 hours; inoculate the Trichoderma viride strains that have been activated and cultured into a fermenter with a volume ratio of 70% fermentation medium, and the inoculum volume is 3% by volume , the formula of the fermentation medium is glucose 12g / l, corn cob 6g / l, calcium chloride 0.6g / l, magnesium sulfate 0.5g / l, potassium dihydrogen phosphate 22g / l, yeast extract 12g / l, sulfuric acid Ferrous 7mg / l, zinc sulfate 1.8mg / l, cobalt chloride 4.0mg / l and manganese sulfate 1.8mg / l, at a culture tempe...
Embodiment 3
[0061] High-density culture of Trichoderma viride, Bacillus subtilis, Rhizopus niger, photosynthetic bacteria, Saccharomyces cerevisiae and Bacillus licheniformis:
[0062] (1) Trichoderma viride: inoculate the Trichoderma viride strain on the purified slant medium into a shaker flask filled with an activated medium with a volume ratio of 20%, and the activated medium is wort potato sucrose medium , at a culture temperature of 30°C and a shaker flask rotation speed of 160rpm, activate and cultivate for 36 hours; inoculate the Trichoderma viride strains that have been activated and cultured into a fermenter with a volume ratio of 60% fermentation medium, and the inoculum volume is 2% by volume , the formula of the fermentation medium is glucose 10g / l, corn cob 5.5g / l, calcium chloride 0.4g / l, magnesium sulfate 0.3g / l, potassium dihydrogen phosphate 20g / l, yeast extract 10g / l, Ferrous sulfate 5mg / l, zinc sulfate 1.5mg / l, cobalt chloride 3.7mg / l and manganese sulfate 1.6mg / l, at ...
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