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Chicken infectious coryza inactivated vaccine adjuvant

A chicken infectious rhinitis and inactivated vaccine technology, applied in the field of chicken infectious rhinitis inactivated vaccine adjuvants, can solve the problems of low antibody titer, side effects, short duration, etc.

Inactive Publication Date: 2012-04-11
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The oil emulsion inactivated vaccine antibody lasts longer, but there are different degrees of side effects
The inactivated vaccine with aluminum gel adjuvant has no side effects, but the antibody titer is low and the duration is short

Method used

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  • Chicken infectious coryza inactivated vaccine adjuvant
  • Chicken infectious coryza inactivated vaccine adjuvant
  • Chicken infectious coryza inactivated vaccine adjuvant

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0024] (3) Preparation of seedling bacteria solution

[0025] 1) Bacterial solution culture. Add 100ml seed solution to 4000ml semi-synthetic medium, culture at 37°C for 18-20 hours, indirectly shake the culture flask twice, and confirm that there is no bacteria contamination after pure picking, add 0.05% formaldehyde solution, and place Store at 2~8℃.

[0026] 2) Concentration and inactivation of the bacterial solution for seedling production Use a hollow fiber ultrafilter to concentrate the purely qualified bacterial solution, and then use pH7.2 PBS to make a suspension, so that each 1ml contains at least 5 billion bacterial cells (using biological The national standard product "China Bacterial Turbidity Standard" is compared. The standard tube contains Haemophilus paragallinarum (2.8 billion / ml), and then 0.15% formaldehyde solution and 0.01% thimerosal are added by volume at 2~8℃. Sterilized for 7 days and tested aseptic growth as seedling antigen.

[0027] (4) Preparation of a...

Embodiment 1

[0040] 1 strain

[0041] The strain used for manufacturing and testing is Haemophilus paragallinarum type A C-Hpg-8 strain, which is identified, kept and supplied by the China Veterinary Drug Administration.

[0042] 2 Vaccine manufacturing

[0043] (1) Preparation of seeds for production

[0044] 1) Propagation and identification of first-class seeds: Take the strains and inoculate them on chicken broth agar plates with 5% to 10% CO 2 After culturing at 37°C for 16 to 18 hours, select several typical lawns (colony) with strong fluorescence and inoculate them in the yolk sacs of 5-day-old chicken embryos. Continue incubating at 37°C and collect egg yolks that died within 30 hours. The liquid, after passing the pure inspection, is used as the-grade seed. It should be stored below -20℃ for no more than 1 month, otherwise it will be rejuvenated by chicken embryos, but it should not exceed 6 generations.

[0045] 2)Secondary seed reproduction: Take the infected chicken embryo egg yolk liq...

Embodiment 2

[0060] According to "Manufacturing and Inspection Regulations of Inactivated Avian Infectious Rhinitis Vaccine" (The Ministry of Agriculture of the People's Republic of China. The Regulations for Veterinary Biological Products of the People's Republic of China, 2000 Edition. Chemical Industry Press, 2000, the present invention is hereinafter referred to as "the Regulations") The test method on the vaccine is tested.

[0061] 1. Sterility inspection is carried out in accordance with the "Procedures", and grows aseptically.

[0062] 2. Safety inspection 8 healthy and susceptible chickens aged 2 to 3 months were injected subcutaneously with 1ml vaccine. Observed for 14 days, there should be no abnormal reactions.

[0063] 3. Efficacy test Use 8 healthy and susceptible chickens aged 2 to 3 months, each of which is injected subcutaneously with 0.5ml vaccine. One month later, together with 4 control chickens with the same conditions, each suborbital sinus was injected with 0.2ml of C-Hp8-...

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Abstract

The invention relates to a chicken infectious coryza inactivated vaccine adjuvant. The adjuvant prepared in the invention is used as a chicken infectious coryza inactivated vaccine adjuvant to prepare a chicken infectious coryza inactivated vaccine and has the advantages of high antibody titer produced by inoculated chicken, long antibody protection period, high toxin expelling protection rate and the like.

Description

Technical field [0001] The invention relates to an avian infectious rhinitis inactivated vaccine adjuvant, which belongs to the field of biology, especially the field of veterinary biological products. Background technique [0002] There are two main types of inactivated rhinitis vaccines currently on the market, including oil adjuvant and aluminum gel adjuvant vaccine. The oil emulsion inactivated vaccine antibody lasts for a long time, but there are different degrees of side effects. Aluminum gel adjuvant inactivated vaccine has no side effects, but the antibody titer is low and the duration is short. [0003] The inactivated chicken infectious rhinitis vaccine prepared by the novel adjuvant of the present invention has the advantages of high safety, no side effects, fast antibody production and high protection rate. It is not only suitable for routine immunization of chickens, but also effective for preventing epidemics. Occurrence, and is suitable for emergency vaccination of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61P31/04A61K39/102
Inventor 韩建文周忠号李平杜元钊
Owner YEBIO BIOENG OF QINGDAO
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