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Grifola frondosa polysaccharide ZZK component and preparation method thereof

A technology of Grifola frondosa polysaccharide and flower seeds, which is applied in the directions of medical preparations, drug combinations, and pharmaceutical formulas containing active ingredients.

Active Publication Date: 2012-04-11
HANGZHOU ZHONGZHI KANGGU BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The Grifola frondosa polysaccharide ZZK component of the present invention is a kind of β-glucomannan (ZZK) obtained by separating the frondosa frondosa fruit body as raw material through drying, pulverization, extraction, purification and other processes. Not reported in the literature

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The preparation of embodiment 1 Grifola frondosa polysaccharide ZZK component

[0051] Grifola frondosa dry fruiting body 1 kg, dried in a blast drying oven at 50°C for 5 hours, pulverized into fine powder with a powder machine, and passed through a No. 5 sieve to obtain 0.98 kg of fine powder. Add 10L of hot water to the fine powder, stir evenly, boil and extract for 2 hours, filter the extract with a plate and frame to remove the residue to obtain a clarified extract, and extract the extract with an ultrafiltration membrane with a cut-off weight average molecular weight of 800,000, which can pass through the membrane The part (weight-average molecular weight is less than 800,000) is then concentrated by ultrafiltration with an ultrafiltration membrane with a weight-average molecular weight cut-off of 200,000, and the part that cannot pass through 200,000 (weight-average molecular weight is greater than 200,000) is the concentrate. Add 2 times the volume of absolute et...

Embodiment 2

[0052] Preparation of Example 2 Grifola frondosa polysaccharide ZZK component

[0053] Grifola frondosa fruit body fresh product 10 kg, dried in a blast drying oven at 60°C for 15 hours, pulverized into fine powder with a powder machine, and passed through a No. 5 sieve to obtain 1.2 kg of fine powder. Add 12L of hot water to the fine powder, stir evenly, boil and extract for 2 hours, filter the extract with a plate and frame to remove the residue to obtain a clarified extract, and extract the extract with an ultrafiltration membrane with a cut-off weight average molecular weight of 800,000, which can pass through the membrane The part (weight-average molecular weight is less than 800,000) is then concentrated by ultrafiltration with an ultrafiltration membrane with a weight-average molecular weight cut-off of 200,000, and the part that cannot pass through 200,000 (weight-average molecular weight is greater than 200,000) is the concentrate. Add 2 times the volume of absolute e...

Embodiment 3

[0054] Example 3 Structural Analysis of Grifola frondosa Polysaccharide ZZK Component

[0055] Three batches of ZZK components in Example 1 (batch numbers 20100401, 20100402, 20100402) and 3 batches of ZZK components in Example 2 (batch numbers 20100501, 20100502) were taken for structural analysis. The monosaccharide composition analysis adopts PMP (1-phenyl-3-methyl-5-pyrazolone) derivatization method; the connection mode of monosaccharide is analyzed by partial methylation and partial acetylation method; the polysaccharide configuration is analyzed by infrared analysis method; protein detection adopts ultraviolet scanning; weight average molecular weight is measured by high performance liquid gel permeation chromatography (GPC), and the detector uses differential detector; according to the provisions of the second part of the Chinese Pharmacopoeia, the specific rotation of 5 batches of ZZK components is determined according to law , The measured temperature is 20°C. 5 batc...

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Abstract

The invention relates to a Grifola frondosa polysaccharide ZZK component and a preparation method thereof. The fruit body in Grifola frondosa is used as the raw material and is subjected to drying, crushing, extraction, purification and other processes, thus being separated into a beta glucomannan (ZZK). The beta glucomannan (ZZK) comprises the main monosaccharides of glucose and mannitose, has a weight-average molecular weight of 380-750 thousand Daltons and has remarkable antineoplastic activity.

Description

(1) Technical field [0001] The invention relates to a Grifola frondosa polysaccharide ZZK component and a preparation method thereof. Grifola frondosa fruit bodies are crushed, extracted and other steps are taken to obtain a Grifola frondosa polysaccharide ZZK component, which is mainly composed of glucose and mannose, and can be used to make food additives and various pharmaceutical preparations. The invention belongs to the fields of food processing and pharmaceutical industry. (2) Background technology [0002] Since the 1980s, scholars at home and abroad have conducted a lot of research on the polysaccharides of Grifola frondosa, and extracted dozens of active polysaccharides from the fruiting body and mycelia of Grifola frondosa, including D-component, MD component, Grifolan Components, T-2 components and other components with significant physiological activity. The common feature of these components is that they are composed of β-1,3-linked and β-1,6-linked glucose, ...

Claims

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Application Information

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IPC IPC(8): C08B37/02A61K31/736A61P35/00
Inventor 毛延卿杨勇杰张萍郑宝芬杨小丽
Owner HANGZHOU ZHONGZHI KANGGU BIOLOGICAL TECH
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