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Detection method of glaucescent fissistigma root saponins

A black bone vine and determination method technology, applied in the field of biomedicine, can solve the problems of uncontrollable chemical composition content, large error, poor specificity, etc., and achieve the effect of overall quality, good recovery rate, and high separation degree

Inactive Publication Date: 2012-04-18
JIANGSU TIANSHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

"Determination of Tenacissoside B Content in Aegypti Aegypti by RP-HPLC Method" published in the second issue of "Chinese Journal of Traditional Chinese Medicine" in 2010, disclosed a reverse-phase high-performance liquid chromatography method for detection of Aegypti saponin B As for the method of content, the detector selected is an ultraviolet detector, and since acacia saponin has no characteristic absorption in the ultraviolet region, so the detection of this method must have the disadvantages of poor specificity and large error
Li Huali, Lu Yan, Hu Jiayun, and Chen Daofeng published "HPLC-ELSD Determination of the Content of Guanteng Glycoside H in Tengguanteng" published in "Chinese Journal of Traditional Chinese Medicine" in the 16th issue of 2010. The content of the method, the method is only for the detection of saponin H in the vine medicinal material of the vine, and cannot control the content of other chemical components

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1 Chromatographic conditions and system adaptability

[0023] Chromatographic column: C18 chromatographic column (WondaSilTM 4.6 × 150mm, 5 μ m); mobile phase is acetonitrile: water (38: 62), column temperature 30 ℃; flow rate 1ml / min; evaporative light dispersion detector attenuation is set to 5, atomization The temperature is 30°C, the evaporation temperature is 45°C, and the gas pressure is 22psi. The detection environment temperature is 25°C and the relative humidity is 35%.

[0024] 2 Experimental methods and results

[0025] 2.1 Preparation of reference solution:

[0026] Accurately weigh 9.67mg of the reference substances acaciaside A, 8.79mg of acaciaside B, 9.14mg of acaciaside D, and 8.99mg of acaciaside H, put them in a 10ml measuring bottle, dissolve them in methanol and make The following two groups contain mixed reference solution of different concentrations of acacia saponins A, B, D, H respectively.

[0027] The first group: (1) 96.7μg / ml, (2) 87.9μg...

Embodiment 2

[0036] 1 Chromatographic conditions and system adaptability

[0037] Chromatographic column: C18 chromatographic column (WondaSilTM 4.6 * 150mm, 5 μ m); mobile phase is methanol: water (30: 70), column temperature 30 ℃; flow rate 0.8ml / min; evaporative light dispersion detector attenuation is set to 4, mist The melting temperature is 35°C, the evaporation temperature is 50°C, and the gas pressure is 26psi. The detection environment temperature is 25°C and the relative humidity is 30%.

[0038] 2 Experimental methods and results

[0039] 2.1 Preparation of reference substance solution: same as Example 1.

[0040] 2.2 Preparation of the test solution:

[0041] Accurately weigh 10 mg of the content of Acacia saponin capsules (batch number: 20101109), put it in a 10ml measuring bottle, add a certain amount of methanol to dissolve, after ultrasonication for 20 minutes, let it cool, filter, and dilute to the mark with methanol to obtain

[0042] 2.3 Content determination

[004...

Embodiment 3

[0047] 1 Chromatographic conditions and system adaptability

[0048] Chromatographic column: C18 chromatographic column (WondaSilTM 4.6 × 150mm, 5 μ m); mobile phase is acetonitrile: water (44: 56), column temperature is 30 ℃; The temperature is 30°C, the evaporation temperature is 45°C, and the gas pressure is 24psi. The detection environment temperature is 25°C and the relative humidity is 40%;

[0049] 2 Experimental methods and results

[0050] 2.1 Preparation of reference substance solution: same as Example 1.

[0051] 2.2 Preparation of the test solution:

[0052] Accurately weigh 30 mg of acacia saponin (batch number: 20101112), put it in a 10 ml measuring bottle, add a certain amount of acetonitrile: water (44:56) to dissolve, and after ultrasonication for 15 minutes, let cool, let cool, filter, and use acetonitrile: Dilute water (44:56) to the mark, and you get it.

[0053] 2.3 Content determination

[0054] Accurately draw 20 μl of the reference substance solut...

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Abstract

The invention provides a detection method of glaucescent fissistigma root saponins. The HPLC-ELSD (High Performance Liquid Chromatography-Evaporative Light-Scattering Detector) method established by the invention can be used for simultaneously detecting the contents of glaucescent fissistigma root saponin A, glaucescent fissistigma root saponin B, glaucescent fissistigma root saponin D and glaucescent fissistigma root saponin H, has good precision and reproducibility, and can accurately and completely control the quality of the glaucescent fissistigma root saponins.

Description

technical field [0001] The invention relates to a method for detecting various active ingredients in acacia saponin capsules, belonging to the field of biomedicine. Background technique [0002] Tumor is the number one killer that endangers human life identified by the World Health Organization, and its incidence has shown an overall upward trend in recent years. At present, most antineoplastic drugs are obtained through chemical synthesis. Although the curative effect is good, the side effects are very serious. Therefore, it is of great social significance to develop high-efficiency, low-toxic natural antitumor drugs. [0003] Wuguteng, also known as Tongguangteng Marsdenia tenacissima (Roxb.) Wight et Arn., was first recorded in "Southern Yunnan Materia Medica". It has a bitter taste, slightly cold, clearing heat and detoxifying, dispelling stagnation and relieving pain, etc. It has good clinical anti-tumor effects Curative effect, the Xiaoaiping preparation produced by ...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 易铭钟慧陈月红朱海亮杨永安季浩
Owner JIANGSU TIANSHENG PHARMA
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