Enhanced fluorescence thin layer identification method for Cyperus Rotundus L
A technology of thin-layer identification and fluorescence enhancement, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of endangering the health of patients, the inability to monitor the quality of preparations in an all-round way, and the low increase rate of thin-layer identification, so as to achieve the goal of increasing the increase rate Effect
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Embodiment 1
[0067] Thin-layer identification of toad venom by fluorescence detection:
[0068] a. Take 0.05 g of toad venom medicinal material and toad venom reference medicinal material powder respectively, add 3 milliliters of methanol, sonicate for 15 minutes, and take the supernatant as the test solution and the reference solution;
[0069] b. Take 2 microliters each of the test solution and the reference solution, and place them on the same GF respectively 254 On the thin-layer plate, develop with cyclohexane-ethyl acetate-formic acid with a volume ratio of 4:6:0.2 as the developer, take it out, dry it, and inspect and take pictures under 254nm and 365nm ultraviolet lamps respectively;
[0070] c. Spray 10% sulfuric acid ethanol solution as a fluorescence enhancer on the thin-layer plate, and heat at 105°C until the spots are clearly colored;
[0071] d. Check and take pictures under 365nm ultraviolet light;
[0072] see results figure 1 , it can be seen that after the fluorescen...
Embodiment 2
[0074] TLC identification of Atractylodes macrocephala by fluorescence detection:
[0075] a. Take 0.5 g of Atractylodes macrocephala medicinal material and Atractylodes macrocephala reference medicinal material powder respectively, add 5 milliliters of methanol, sonicate for 10 minutes, and take the supernatant as the test solution and the reference solution;
[0076] b. Take 4 microliters each of the test solution and the reference solution, and place them on the same GF respectively. 254 On the thin-layer plate, develop with cyclohexane-ethyl acetate-formic acid with a volume ratio of 8:2:0.2 as the developer, take it out, dry it, and inspect and take pictures under 254nm and 365nm ultraviolet lamps respectively;
[0077] c. Spray 10% sulfuric acid ethanol solution as a fluorescence enhancer on the thin-layer plate, and heat at 105°C until the spots are clearly colored;
[0078] d. Check and take pictures under 365nm ultraviolet light;
[0079] see results figure 2 , it...
Embodiment 3
[0081] TLC identification of forsythia by fluorescence detection:
[0082] a. Take 0.4 g of Forsythia medicinal material and Forsythia reference drug respectively, add 5 milliliters of methanol, ultrasonically treat for 10 minutes, and take the supernatant as the test solution and the reference solution;
[0083] b. Take 4 microliters each of the test solution and the reference solution, and place them on the same GF respectively. 254 On the thin-layer plate, develop with cyclohexane-ethyl acetate-formic acid with a volume ratio of 10:2:0.2 as the developer, take it out, dry it, and inspect and take pictures under 254nm and 365nm ultraviolet lamps respectively;
[0084] c. Spray 10% sulfuric acid ethanol solution as a fluorescence enhancer on the thin-layer plate, and heat at 105°C until the spots are clearly colored;
[0085] d. Check and take pictures under 365nm ultraviolet light;
[0086] see results image 3 , it can be seen that after the fluorescence is increased, th...
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