Method for extracting 5-hydroxytryptophan

Abstract

The invention relates to a method for extracting 5-hydroxytryptophan. The method has the advantages that: the yield of the product is improved and the energy consumption is reduced because an antioxidant is added to protect and an ion exchange resin and a macroporous resin are combined. The method comprises the following steps of: crushing a raw material, namely griffonia seeds, soaking the raw material into acidic water and sodium pyrosulfite for extraction for 2 to 3 times, wherein the amount of the acidic water and the amount of the sodium pyrosulfite are both 8 to 15 times that of the raw material and the raw material is soaked for 4 to 10 hours at each time, adsorbing the extract by adding the extract into a cation exchange resin column, washing the extract with deionized water until the extract is colorless, eluting the extract with aqueous ammonia (containing 0.1 to 0.3 percent of sodium metabisulfite) of which the concentration is 10 to 15 percent, adjusting the pH value of the eluate to 2 to 4, adsorbing the eluate by adding the eluate into a macroporous resin column, washing the eluate with water until the eluate is neutral, eluting the effective component with 4-6 BV ethanol solution of which the concentration is 40 to 60 percent, collecting the eluate, adding the eluate into active carbon to decolor the eluate, reclaiming the ethanol, concentrating the decoloring liquid until the concentration of the decoloring liquid is 1.4 to 1.6, placing the decoloring liquid until the decoloring liquid is crystallized, filtering the crystal, and drying the crystal to obtain the product. The method is simply operated and is suitable for industrial production.

Description

Technical field:

[0001] The invention belongs to the field of separation of natural products, in particular to a method for extracting 5-hydroxytryptophan. Background technique:

[0002] 5-Hydroxytryptophan, English name is 5-hydroxytryptopHan, chemical name: 5-hydroxy-3-indolyl-alanine, molecular formula: C 11 h 12 N 2 o 3 , molecular weight: 220.23.

[0003] 5-Hydroxytryptophan is a precursor substance of a hormone called serotonin in the human body, which can help the production of this hormone in the human body. 5-Hydroxytryptophan is an important inhibitor of neurotransmitter serotonin (5-HT ) precursor. There are serotonin in the brain, platelets, stomach, and central nervous system. When the mood changes, serotonin will be secreted accordingly. It is an important chemical substance in the brain to control emotions, behaviors, appetite, sleep, and impulse. 5-hydroxytryptamine can effectively help produce serotonin, so it can promote the health of mood and nervous...