Bone tissue constructed by tissue engineering

A tissue engineering and bone tissue technology, applied in the field of biomedical engineering, can solve the problems of lack of bone mineral, lack of basic mechanical stiffness, lack of basic mechanical strength, etc., and achieve the effect of good biocompatibility

Inactive Publication Date: 2012-05-30
DALIAN CHUANGDA TECH TRADE MARKET
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The former does not contain organic components, so it is loose and brittle, and does not have basic mechanical strength; the latter lacks bone mineral, so its texture is soft and does not have basic mechanical rigidity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] l) In vitro isolation and culture of bone marrow mesenchymal stem cells: extract 5-10 ml of the patient's own bone marrow, separate with Ficoll, extract nucleated cells, and wash twice with PBS + EDTA. Add DMEM (purchased from GIBCOBRL company. Contains 20% fetal bovine serum) culture medium, place in fibronectin-coated culture dish, 37 ℃ containing 5% carbon dioxide incubator. After two days, unattached cells were removed and cultured in conditioned medium.

[0028] Subculture

[0029] When the cells are confluent to 60%-75% density, rinse with 10ml of calcium and magnesium-free PBS, digest with 0.25% trypsin at 37°C for 5 minutes, add DMEM culture solution containing 10% fetal bovine serum to stop the digestion, transfer to a centrifuge tube, Centrifuge at 1200r / min for 5 minutes. Discard the supernatant, add 10ml of DMEM culture solution containing 10% fetal bovine serum, mix well, count, and subculture at a ratio of 1:3. Change the medium every 2-3 days until the...

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PUM

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Abstract

The invention belongs to the field of medical engineering, particularly relates to a bone tissue constructed by tissue engineering. Tissue engineered bone scaffold materials are shaped according to specific defect forms of bones required to be restored. The outer layer of the shaped tissue engineered bone is a bone cortical frame, cancellous bone substitutes inoculated with bone marrow mesenchymal stem cells are filled in the middle of the shaped tissue engineered bone and substitute for cancellous bone and bone marrow compositions in the normal bone structure. The tissue engineered bone comprises cortical bone, cancellous bone and cell compositions which are similar to those of the normal bone structure. In clinical application, doctors can determine the resection range in advance according to CT (computed tomography) films of patients in operations, and shape the tissue engineered bone scaffold materials according to CT reconstruction data of the bone defect portions, thereby realizing individual treatment.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, and in particular relates to a bone tissue constructed through tissue engineering. Background technique [0002] Tissue engineering is a hot spot in life science in the 21st century. Its core content is three-dimensional cell culture in vitro, and its basic method is to form a complex of seed cells and biodegradable biomaterials to replace damaged tissues and organs. At present, autologous bone is mainly used as bone tissue substitutes at home and abroad, accounting for about 70%. Relatively successful in xenograft bone are some bone mineral materials that completely remove the organic components of bone, such as KEIL bone, BIOOSS bone, and inorganic bone, and organic component materials that completely remove bone mineral components, such as demineralized bone, bone matrix gelatin, etc. The former does not contain organic components, so it is loose and brittle, and does not have basic me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/36A61L27/12
Inventor 杜冲
Owner DALIAN CHUANGDA TECH TRADE MARKET
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