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Method for detecting action mechanism of ursolic acid in inhibiting AGEs (advanced glycation end products)

A technology of inhibition and mechanism of action, applied in the field of pathological detection, can solve problems such as unclear mechanism of action

Inactive Publication Date: 2012-05-30
SUZHOU HEALTH COLLEGE
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  • Abstract
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Problems solved by technology

[0003] Ursolic acid (UA) has various biological effects such as anti-tumor, anti-diabetes, lipid-lowering, and anti-oxidation. Our previous animal experiments have confirmed that UA can improve blood vessel damage in DM, but its mechanism is still unclear

Method used

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  • Method for detecting action mechanism of ursolic acid in inhibiting AGEs (advanced glycation end products)

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Embodiment Construction

[0010] A method for detecting the mechanism of action of ursolic acid inhibiting AGEs, comprising the following steps:

[0011] Step 1: Simulate the in vivo protein non-enzymatic glycosylation reaction system;

[0012] Based on the three factors of bovine serum albumin (BSA) concentration, glucose concentration and incubation time, with four levels for each factor, the optimal conditions were screened with the L16(45) orthogonal test reaction system.

[0013] Add BSA to the PBS solution to make the final mass concentration of 1, 2, 2.5, 5g / L, d-glucose mass final concentration of 5, 10, 25, 50mM, add 10mM EDTA antiseptic, 0.2m micro After aseptic filtration by the pore filter membrane, place in a 37°C incubator and incubate for 20, 40, 60, 80 days, forming the table 1 orthogonal test factor level table.

[0014] Table 1 Orthogonal test factor level table

[0015]

[0016] When the incubation reaches the set time, samples are taken, and the fluorescence value of glycated a...

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Abstract

The invention discloses a method for detecting an action mechanism of ursolic acid in inhibiting AGEs (advanced glycation end products), which includes the steps: firstly, simulating a non-enzymatic glycation system of body proteins; secondly, detecting inhibition of the ursolic acid against generation of fructosamine; thirdly, detecting inhibition of the ursolic acid against generation of AGEs; fourthly, processing data, statistically comparing inhibition of the ursolic acid against generation of the fructosamine to inhibition of the ursolic acid against generation of AGEs, and obtaining the action mechanism of the ursolic acid in inhibiting AGEs. The method can be used for simply and effectively detecting the action mechanism of the ursolic acid in inhibiting AGEs.

Description

technical field [0001] The invention belongs to the field of pathological detection, in particular to a method for detecting the mechanism of action of ursolic acid inhibiting AGEs. Background technique [0002] The persistent hyperglycemia state in the human body will lead to non-enzymatic glycation reactions between many structural and functional proteins and nucleic acid proteins and glucose to form advanced glycation products (Advance Glycation End Products, AGEs). AGEs can not only affect the normal structure and function of these proteins In addition, it can also play a variety of pathological roles through the receptor for advanced glycation (RAGE). It has been proved that AGEs are one of the main reasons for the accelerated occurrence of vascular disease in patients with diabetes (Diabetic Mellitus, DM). Therefore, inhibiting the formation of AGEs is an important means to prevent and treat diabetic vascular disease. [0003] Ursolic acid (UA) has various biological ...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/64
Inventor 王建梅向敏张雅琴吴萍萍
Owner SUZHOU HEALTH COLLEGE
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