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Reaction Medium For Methicillin-resistant Staphylococcus Aureus (mrsa) Bacteria

A technology for methicillin resistance and staphylococci, which is applied in the determination/testing of microorganisms, biochemical equipment and methods, etc., and can solve the problems of reducing medium utilization and increasing costs

Inactive Publication Date: 2012-05-30
BIOMERIEUX SA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the detection of phosphatase activity is not very specific under the conditions described, it needs to be combined with the detection of several other enzyme activities, thereby reducing the utilization of the medium and increasing the cost

Method used

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  • Reaction Medium For Methicillin-resistant Staphylococcus Aureus (mrsa) Bacteria
  • Reaction Medium For Methicillin-resistant Staphylococcus Aureus (mrsa) Bacteria
  • Reaction Medium For Methicillin-resistant Staphylococcus Aureus (mrsa) Bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080]Embodiment 1 comprises the MRSA culture medium of cefminox-kanamycin antibiotic combination and various chromogenic substrates

[0081] 1. Prepare the culture medium of the present invention

[0082] The media tested in the experiments were as follows:

[0083] Medium T: chromID MRSA control medium (ref.43451).

[0084] Medium A: Medium T; Cefoxitin is replaced by cefminox (Daewoo Chemical, A8060902; 16 mg / l) / kanamycin (Sigma, ref. K4000; 1 mg / l) antibiotic combination; the chromogenic substrate is Pink - Phosphate (Biosynth, ref. C-5100; 0.25 g / l).

[0085] Medium B: Medium T; Cefoxitin is replaced by cefminox (16mg / l) / kanamycin (0.75mg / l) antibiotic combination; Chromogenic substrate is X-α-glucoside (Biosynth, B7230 ; 0.045 g / l) and Green A-α-glucoside (Inalco, ref. 1758-0730).

[0086] Medium C: Medium T; Cefoxitin is replaced by cefminox (16mg / l) / kanamycin (0.75mg / l) antibiotic combination; Chromogenic substrate is X-α-glucoside (Biosynth, B7230 ; 0.045 g / l) an...

Embodiment 2

[0093] Embodiment 2 comprises the MRSA culture medium of the cefminox of various concentrations and kanamycin

[0094] 1. Prepare the culture medium of the present invention

[0095] The media tested in the experiments were as follows:

[0096] Medium T: chromID MRSA control medium (ref.43451).

[0097] Medium D: Medium T; Cefoxitin is replaced by various concentrations of cefminox (the same antibiotic used in Example 1) / kanamycin (the same antibiotic used in Example 1) antibiotic combination; color development The substrates were X-α-glucoside (same substrate as used in Example 1; 0.045 g / l) and Green A-α-glucoside (same substrate as used in Example 1; 0.08 g / l).

[0098] [Cefminol], mg / l

[Kanamycin], mg / l

D1

16

0.75

D2

16

1

D3

14

0.75

D4

14

1

[0099] 2. Media Inoculation and Reading

[0100] Carry out this step as embodiment 1

[0101] 3. Results

[0102] The resu...

Embodiment 3

[0105] Embodiment 3 comprises the MRSA culture medium of cefminol-amikacin antibiotic combination and various chromogenic substrates

[0106] 1. Prepare the culture medium of the present invention

[0107] The media tested in the experiments were as follows:

[0108] Medium T: chromID MRSA control medium (ref.43451).

[0109] Medium E: Medium T; Cefoxitin was replaced by cefminox (same antibiotic as used in Example 1; 16mg / l) / amikacin (Sigma, ref.2324; 2mg / l) antibiotic combination; The color substrate is pink-phosphate (Biosynth, C-5100; 0.25 g / l).

[0110] Medium F: Medium T; Cefoxitin is replaced by cefminol (16mg / l) / amikacin (2.5mg / l) antibiotic combination; Chromogenic substrate is X-alpha-glucoside (with embodiment 1; 0.045 g / l) and Green A-α-glucoside (same substrate as used in example 1; 0.08 g / l).

[0111] 2. Inoculation and reading of medium

[0112] Carry out this step as embodiment 3

[0113] 3. Results

[0114] The results obtained are shown in Table 3 belo...

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PUM

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Abstract

The present invention relates to a reaction medium for detecting and / or identifying methicillin-resistant Staphylococcus aureus (MRSA) bacteria, said medium containing a chromogenic substrate, a first antibiotic that belongs to the cephalosporin family, and a second antibiotic that belongs to the aminoside family.

Description

technical field [0001] The present invention relates to a reaction medium for detecting methicillin-resistant Staphylococcus aureus (MRSA). The invention also relates to the use of this culture medium and a method for identifying MRSA bacteria. Background technique [0002] Methicillin-resistant Staphylococcus aureus (MRSA) are strains of Staphylococcus aureus characterized by their resistance to the antibiotic methicillin and resistance to related antibiotics such as oxacillin. MRSA bacteria represent a high percentage of nosocomial infections and often produce serious and potentially fatal health problems. MRSA is an epidemic infection that is very difficult to control, and is often cross-transmitted among patients through medical staff. Screening for carriers of MRSA and isolating infected patients constitutes the most effective method currently recommended by official organizations such as the Society for Healthcare Epidemiology of America, besides appropriate treatmen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/14
CPCC12Q1/14
Inventor G·赞巴尔迪J-M·罗什
Owner BIOMERIEUX SA
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