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Peptaibol antibacterial peptide compounds and preparation method and application thereof

A compound and antibacterial peptide technology, applied in the field of Peptaibol antibacterial peptide compounds and their preparation, can solve the problems of high cost, low content of antibacterial peptides, consistent spatial structure of natural antibacterial peptides, etc., and achieve the effect of high research and development potential and high yield

Active Publication Date: 2016-04-20
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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AI Technical Summary

Problems solved by technology

At present, antimicrobial peptides instead of antibiotics as feed additives are still in the exploratory stage, and there are still many problems to be solved for large-scale application, mainly including: the content of antimicrobial peptides in animals is extremely low, and it is difficult to use microorganisms for production due to their broad-spectrum antibacterial effects; Chemical synthesis is expensive and cannot be produced on a large scale; antimicrobial peptides obtained through genetic engineering cannot be guaranteed to be consistent with natural antimicrobial peptides in terms of spatial structure, and it is difficult to ensure the biological activity of recombinant antimicrobial peptides compared with traditional antibiotics; Direct expression of genes in microorganisms, the expression products are often toxic to host cells and cannot be expressed at a high level
These lead to difficult and expensive sources of antimicrobial peptides, which restrict the application of antimicrobial peptides as feed additives

Method used

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  • Peptaibol antibacterial peptide compounds and preparation method and application thereof
  • Peptaibol antibacterial peptide compounds and preparation method and application thereof
  • Peptaibol antibacterial peptide compounds and preparation method and application thereof

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Embodiment 1

[0028]Acremonium persicinum (Acremonium persicinum) SC0105 bacterial strain was inoculated on wheat solid culture medium (add wheat 50g in every 50mLYMG medium, mix and sterilize the gained. Described YMG medium, its preparation method is: mix 4g glucose, 10 g of malt extract and 4 g of yeast extract were dissolved in 1 L of water.) and cultured statically for 16 days at 25° C. without light to obtain a solid fermentation culture.

[0029] Extract the solid fermentation culture with a 95% ethanol aqueous solution for 3 times, each time for 48 hours, and concentrate the ethanol extract under reduced pressure to obtain an extract, which is dissolved in a methanol aqueous solution with a volume fraction of 40%. Macroporous adsorption resin (HP-20) column, with methanol-water mixed solvent as the eluent, eluted from the gradient of methanol-water volume ratio 30:70, 80:20, 100:0, combined methanol-water volume Fraction 1 was obtained from the elution ratio of 80:20, and fraction 1...

Embodiment 2

[0059] Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) were used as test strains, cultivated with 25mL of MHB medium on a shaker at 37°C and 150rpm for 12h, and adjusted the concentration of each bacterial suspension with MHB 1×10 5 CFU / mL. Each test sample (compound 1-4) was dissolved in DMSO and diluted to the required concentration. The final concentration of each test sample was: 200, 100, 50, 25, 12.5, 6.25, 3.125 and 1.5625ug / mL. The same concentration of DMSO was set instead of the test sample as a negative control, AlamarBlue suspension without bacteria was used as a blank control, and kalamycin and vancomycin were respectively used as positive controls. Add 100 μL of bacterial suspension containing AlamarBlue (8%, v / v) and diluted test samples (4%, v / v) to a 96-well plate, and each treatment is repeated three times, at 37°C in the dark incubate. When the color in the well changes from pink to blue, measure the OD value of each we...

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Abstract

The invention discloses Peptaibol antibacterial peptide compounds and a preparation method and application thereof. The four novel Peptaibol antibacterial peptide compounds are separated and obtained from acremonium persicinum SC0105 fermentation products, and it is shown through antibacterial tests that the antibacterial peptide compounds have a remarkable inhibition effect on staphylococcus aureus (MSSA) and methicillin-resistant staphylococcus aureus (MRSA). Peptaibol antibacterial peptide is a main metabolic product of a strain, the yield is high, and high research and development potential is achieved. The Peptaibol antibacterial peptide can serve as an antibiotic substitute and be applied to medicine for preparing antibacterial drugs and feed additives in the breeding industry.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a class of Peptaibol antibacterial peptide compounds and a preparation method and application thereof. Background technique [0002] The emergence of antibiotics has had a profound impact on human defense against bacterial infectious diseases. However, long-term or high-dose antibiotic abuse, including extensive use in the aquaculture industry, on the one hand causes antibiotic residues in food, resulting in potential food safety On the other hand, drug-resistant bacteria are gradually increasing, and there are even superbugs that cannot be killed by existing antibiotics. In April 2014, WHO released the global antimicrobial resistance report for the first time. Among the nine commonly used antimicrobial data from 114 countries, 82 countries developed resistance to more than five antimicrobials, indicating that the problem of antimicrobial resistance is increasing in all reg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C12P21/02C07K1/22C07K1/20C07K1/16A61K38/10A61P31/04C12R1/645
CPCA61K38/00C07K7/08
Inventor 吴萍魏孝义姚磊薛璟花
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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