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Method for extracting flavones in Chinese herbal medicine by surfactant cooperated supersonic wave-enzymatic hydrolysis

A technology of surfactant and Chinese herbal medicine, applied in the field of extracting flavonoids, can solve the problems of easy destruction of heat-sensitive substances, large amount of solvent used, low extraction rate, etc., and achieve good antioxidant properties, enhanced penetration, and flavonoid yield. high effect

Inactive Publication Date: 2014-09-24
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

They all have the disadvantages of large amount of solvent used, long time-consuming, low extraction rate, etc.
Among them, the decoction method and the Soxhlet extraction method also have the problem that heat-sensitive substances are easily destroyed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Extraction of flavonoids from red vine:

[0018] (1) Put 10 g of uncrushed red vine into a three-necked flask, add glucose, cellulase and 100 mL of deionized water, and sonicate at 50 ° C for 10 min;

[0019] The concentration of glucose is 10g / L, and the concentration of cellulase is 0.5g / L;

[0020] The ultrasonic power is 180W, and the ultrasonic frequency is 53kHz;

[0021] (2) Add Tween-60 and continue ultrasonic extraction at 50°C for 20 minutes;

[0022] The concentration of Tween-60 in water is 16g / L;

[0023] The ultrasonic power is also 180W, and the frequency is 53kHz;

[0024] (3) With the extract obtained in step (2), use ultraviolet-visible spectrophotometry to measure the flavone concentration at a wavelength of 510nm, and the flavone concentration is 0.029g / L;

[0025] (4) the extract obtained by step (2) is used to measure its oxidation resistance by DPPH method, and the clearance rate of the described extract solution after 30min is 64.9%, while th...

Embodiment 2

[0027] Extract flavonoids from Digupi:

[0028] (1) Put 5.0 g of unground Digupi into a three-necked flask, add glucose, cellulase and 100 mL of deionized water, and sonicate at 50°C for 10 min;

[0029] The concentration of glucose is 8g / L, and the concentration of cellulase is 0.5g / L;

[0030] The ultrasonic power is 180W, and the ultrasonic frequency is 53kHz;

[0031] (2) Add Tween-60 and continue ultrasonic extraction at 50°C for 20 minutes;

[0032] The concentration of Tween-60 in water is 16g / L;

[0033] The ultrasonic power is also 180W, and the frequency is 53kHz;

[0034] (3) With the extract obtained in step (2), use ultraviolet-visible spectrophotometry to measure the flavone concentration at a wavelength of 510nm, and the flavone concentration is 1.4g / L;

[0035] (4) the obtained extract of step (2) is used to measure its oxidation resistance by DPPH method, and the clearance rate of the described extract solution after 30min is 71%, while the clearance rate ...

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Abstract

The invention discloses a method for extracting flavones in Chinese herbal medicine by surfactant cooperated supersonic wave-enzymatic hydrolysis, which comprises the following steps: 1) uniformly mixing a Chinese herbal medicine, an enzyme preparation, glucose and deionized water and placing in an ultrasonic apparatus, setting the ultrasonic temperature, power and frequency, carrying out enzymatic hydrolysis for a while; 2) adding a surfactant with certain concentration in a liquid solid mixture obtained in the step 1) for ultrasonic extraction; 3) cooling the obtained products from the step 2) to the room temperature, carrying out vacuum filtration to obtain the filtrate which is an extract containing the flavones. The operation method and the external environment of the invention the invention has no high requirements on the operation method and the external enviroments. The surfactant is capable of reducing the tension force of the interface and possessing the solubilization effect, the enzyme preparation possesses the wall breaking effect; the supersonic wave possesses the characteristics of mechanical oscillation and cavitation effect, the extraction time can be shortened, the solvent can be saved, the influence of high temperature on the extracted component can be minimized. According to the invention, the advantages of the extraction method are integrated, the flavones yield in the Chinese herbal medicine is high and the inoxidizability is good.

Description

technical field [0001] The invention relates to a method for extracting flavonoids from Chinese herbal medicines. Background technique [0002] In China, after thousands of years of development, traditional Chinese medicine has gradually formed its own perfect system, which is a brilliant treasure of the Chinese clan. Traditional Chinese medicine extraction methods mainly include: decoction method, dipping method, percolation method and Soxhlet extraction method. They all have the disadvantages of large amount of solvent used, long time consumption, and low extraction rate. Among them, the decoction method and the Soxhlet extraction method also have the problem that heat-sensitive substances are easily destroyed. In addition, the components of Chinese herbal medicine are complex, and the content of active ingredients is generally low. Therefore, research and adoption of new Chinese medicine extraction technology is one of the key points in the modernization of Chinese medi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D11/02B01J19/10C07D311/30C07D311/40
Inventor 韩伟叶亚婧雷祖海葛珊珊姬鹏俊金凌杰
Owner EAST CHINA UNIV OF SCI & TECH