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Microbial type time-temperature indicator for low-temperature circulation items

A time temperature and indicator technology, which is applied in time-temperature indication, uses microorganisms to indicate time and temperature field, can solve the problems of no lactic acid bacteria LGG, preparation time temperature indicator, etc., and achieves the effect of low cost, low cost and improved accuracy

Active Publication Date: 2012-06-20
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Time and temperature indicators have not yet been produced using lactic acid bacteria LGG

Method used

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  • Microbial type time-temperature indicator for low-temperature circulation items
  • Microbial type time-temperature indicator for low-temperature circulation items
  • Microbial type time-temperature indicator for low-temperature circulation items

Examples

Experimental program
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Embodiment

[0025] Preparation of PH indicator: Mix 3 parts of 0.1% bromocresol green ethanol solution and 1 part of 0.2% methyl red ethanol solution for later use.

[0026] Preparation of nutritional substrate: mix 10g / L glucose 40g, 2g / L ammonium sulfate 8g, and 5g / L NaCl solution 20g, and adjust the pH value of the mixture to 7.0 by adding 0.1mol / L NaOH solution. Then sterilize and cool for later use.

[0027] Filter and sterilize the pH indicator with an organic microporous membrane, mix it with the nutrient substrate at a mass ratio of 1:100, take 40ml of the mixed solution and pour it into a transparent container with a small mouth, and insert it into the container through the small mouth. The inoculum of lactic acid bacteria LGG at the period is 1% of the volume of the mixed solution, that is, 400 μL of the bacterial solution, and the time and temperature indicator based on the growth and metabolism of lactic acid bacteria LGG is obtained.

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Abstract

A microbial type time-temperature indicator for low-temperature circulation items comprises a time-temperature indicator and a color comparison card. The time-temperature indicator comprises a container, wherein nutrition substrate, a potential of hydrogen (PH) indicating agent and microbes are stored in the container. The nutrition substrate is nutrient substance needed by growth of the microbes and is mixed by 10g / L dextrose, 2g / L ammonium sulfate and 5g / L NaCl solution according to the mass ratio of 1: 0.18-0.23: 0.47-0.52. The PH indicating agent is a methyl red and bromocresol green mixed indicating agent mixed by 72-77 wt% of bromine cresol green ethanol solution with concentration of 0.1% and 23-28 wt% of methyl red ethanol solution with concentration of 0.2%. The PH indicating agent and the nutrition substrate are mixed according to the mass ratio of 1: 98-103. The microbes are lactobacillus GG (LGG). The microbial type time-temperature indicator for the low-temperature circulation items utilizes the growth and metabolism of the LGG to produce acid and utilizes mixed methyl red and bromocresol green as the indicating agent so that variable color changes can be produced, and the purpose of indicating a time-temperature history experienced by foods is achieved.

Description

technical field [0001] The invention relates to the technical field of time-temperature indication, in particular to the technical field of using microorganisms to indicate time and temperature. Background technique [0002] The shelf life of food usually refers to the time that food can be stored at the most suitable average temperature, or the time limit for storing food under the worst conditions. However, due to the unpredictability of temperature in the entire food circulation process, it is difficult to reach a consensus between the predicted shelf life of food and the real circulation period of food. For example, if a food is calibrated to store at a temperature of 2-6°C and has a shelf life of 20 days, if the food is stored at a temperature higher than 6°C, it will cause the food to spoil before the shelf life expires; otherwise, if it is stored If the temperature is 0°C, the storage time of this kind of food will be longer than 20 days. If it is still in accordance...

Claims

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Application Information

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IPC IPC(8): G01N21/80G01K3/04
Inventor 卢立新贾增芹蔡莹
Owner JIANGNAN UNIV
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