Method and system for automatic correction and compensation of stability of microfluidic chip photoelectric detection light source

Abstract

The invention discloses a method and a system for automatic correction and compensation of stability of a microfluidic chip photoelectric detection light source. The method provided by the invention comprises the following steps that 1, a light spectrum transient-state tiny-change prediction model and a light spectrum steady-state tiny-change prediction model are established in a computer by a BPneural network algorithm; 2, a light emitted by a light source orderly passes through an entrance slit, a first concave mirror, a holographic grating, a second concave mirror, an exit slit and a planar mirror, is reflected to a sample in a detection zone of a microfluidic chip, then is gathered by a gathering lens and is received by a photomultiplier; and original spectrum data is transmitted to the computer by multiple A / D converting circuits; and 3, light spectrum transient variable values and light spectrum stable micro-variable values are subtracted from the original spectrum data by the computer so that accurate absorbance data is formed. The method and the system provided by the invention have the advantages that circuit complexity is simplified; energy consumption is reduced; only several parameters need to be acquired and then are directly transmitted to a computer for data correction; and according to different objects needing to be detected by a microfluidic chip, detection precision can be improved flexibly and effectively through change of a data correction algorithm.

Description

technical field [0001] The invention relates to a microfluidic chip photoelectric detection technology, in particular to a microfluidic chip photoelectric detection light source stability automatic correction and compensation method and device. Background technique [0002] For microfluidic chip photoelectric detection technology, the stability of the light source directly affects the accuracy of sensor detection, and the light intensity of the light source will change with changes in temperature, voltage and other factors during the working process, which will further cause the baseline of the spectrum to drift. As a result, the detection data is seriously distorted. There are many factors that affect the stability of the light source. Fluctuations in the voltage or current of the light source, aging of circuit components, external stray light, ambient temperature, and aging of the light source itself may all cause instability of the light source. However, when the energy ...

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Problems solved by technology

This method simplifies the control factors of light source stability, and has the characteristics of direct and fast response. However, for microfluidic chips that require high light source stability, even weak light source fluctuations will cause large detection data. distortion

The feedback adjustment process of the power supply cannot avoid the generation of light source ripples. Therefore, no matter what kind of light source feedback control scheme is used, the influence of light source ripples exists objectively, and it is difficult to achieve real compensation.

Moreover, the traditional method cannot compensate for the distortion of the detection data caused by the decay of the light source due to long-term use

In addition, the circuit feedback strategy is implemented by hardware, which is a fixed method with poor flexibility and high cost

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