Imaging optical microfluid sensing device and method thereof

Abstract

Disclosed are an imaging optical microfluid sensing device and a method thereof. The sensing device comprises a tunable laser light source, a collimating lens, an incidence angle adjusting rack, a reflection prism, an optical microfluid resonant cavity array, a microlens array, an array photoelectric detector, and a signal control and processing unit. Lights emitted from the light source pass through the collimating lens and become parallel light beams, which are incident on the reflection prism. The incident lights at the bottom of the reflection prism are coupled through an evanescent fieldso as to excitate resonant mode of the optical microfluid resonant cavity array for sensing. The reflected lights with the wavelength information of the resonant mode are incident on the array photoelectric detector for imaging. When continuously tuning laser light output wavelength, corresponding multiple-frame images are recorded. Serial processing is carried out on each pixel in the array photoelectric detector in the signal processing unit according to record light source wavelength, so as to realize wavelength sensing querying of each optical microfluid resonant cavity. The apparatus caneffectively realize multichannel optical microfluid sensing and can be used for the construction of a high-flux optical microfluid detection system.

Description

technical field

[0001] The invention relates to the field of various multi-channel multiplexing technologies based on optical micro-resonance sensing, especially the space-division multiplexing of optical microfluidic sensing, which belongs to photobiological and chemical sensing technologies. Background technique

[0002] The label-free biosensor based on microresonant optical technology directly measures molecular interactions, which can realize real-time observation of biomolecular interactions. Since the analyte to be measured does not need special properties such as fluorescence, characteristic absorption or scattering bands, the range of measurement objects is greatly expanded. It can detect toxins, proteins, DNA, and even the entire cell behavior, thus providing a powerful analytical tool for medical diagnosis, drug development, food monitoring, environmental monitoring and other fields.

[0003] The optical microfluidic resonator uses total reflection to completely c...

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