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Freeze-dried powder composition containing Bifidobacterium longum cell lysate and preparation method and application thereof

A technology of Bifidobacterium longum and lysate, which is used in skin care preparations, pharmaceutical formulations, cosmetic preparations, etc., and can solve the problem of preventing aging skin and the combination of Bifidobacterium longum lysate and chicken embryo essence. And other issues

Inactive Publication Date: 2012-07-04
SHANGHAI DELTA IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In addition to the bifidobacterium longum lysate that has been reported in the literature to have the effect of anti-allergic and preventing skin dryness, there is no report on the effect of preventing aging skin, and there is no combination of bifidobacterium longum lysate and chicken embryo essence. Report on Preparation of Anti-aging and Whitening Cosmetics

Method used

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  • Freeze-dried powder composition containing Bifidobacterium longum cell lysate and preparation method and application thereof
  • Freeze-dried powder composition containing Bifidobacterium longum cell lysate and preparation method and application thereof
  • Freeze-dried powder composition containing Bifidobacterium longum cell lysate and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 Preparation of Bifidobacterium longum lysate freeze-dried powder

[0063] Mix and dissolve the freeze-dried powder of Bifidobacterium longum and phosphate buffer according to the mass volume ratio (kg / L) 1:5.0, and then use ultrasonic waves to break the cell wall of Bifidobacterium longum. The ultrasonic program is: ultrasonic 3s, interval 3s, ultrasonic 20 cycles. Follow this procedure for 3 more ultrasounds. After sonication, centrifuge at 6000rpm for 20min. Add trehalose to the supernatant after centrifugation according to the mass volume ratio (kg / L) trehalose: solution = 1:3.0, freeze-dry at -80°C for 18 hours to obtain freeze-dried powder of Bifidobacterium longum.

Embodiment 2

[0064] Example 2 Preparation of Lysate of Bifidobacterium longum and Chicken Embryo Essence Composition Freeze-dried Powder

[0065] Mix and dissolve the freeze-dried powder of Bifidobacterium longum and phosphate buffer according to the mass volume ratio (kg / L) 1:5.0, and then use ultrasonic waves to break the cell wall of Bifidobacterium longum. The ultrasonic program is: ultrasonic 3s, interval 3s, ultrasonic 20 cycles. Follow this procedure for 3 more ultrasounds. After sonication, centrifuge at 6000rpm for 20min. Mix the centrifuged supernatant with the chicken embryo essence at a volume ratio of 1.5:1, and add trehalose to the mixture at a mass-volume ratio (kg / L) of trehalose:mixed solution=1:3.0, Freeze-dry at 80°C for 18 hours to obtain a freeze-dried powder of the composition.

[0066] The following is an in vitro test

Embodiment 3

[0067] Example 3 Antioxidant (free radical scavenging) effect of Bifidobacterium longum lysate and chicken embryo essence composition

[0068] Free radicals are one of the main factors leading to skin aging. Antioxidant substances can combine with or replace free radicals, which reduces the number of [DPPH·] free radicals, and the color of the solution becomes lighter, which is manifested as light absorption at 515 nm The value keeps decreasing until it reaches a stable value. Therefore, the antioxidant or free radical scavenging ability can be calculated (judged) by measuring the ability of antioxidant substances to capture [scavenge] free radicals at 515 nm. It is used for anti-oxidation, anti-aging and whitening effects on functional cosmetic raw materials. [DPPH·] (diphenylpicrylhydrazyl radical) is a relatively stable N-centered radical. [DPPH·]methanol solution is purple, and has the maximum light absorption at 515 nm wavelength, and the depth of the color has a line...

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Abstract

The invention belongs to the technical field of cosmetics and in particular relates to a freeze-dried powder composition containing Bifidobacterium longum cell lysate, a preparation method thereof and application thereof in cosmetics. The freeze-dried powder composition is obtained by the method comprising the following steps of: thoroughly mixing a fermentation culture product of Bifidobacterium longum cells and a phosphate buffer solution at a certain weight / volume ratio, breaking cell walls ultrasonically, centrifuging after ultrasonic treatment to obtain a lysate, adding appropriate amounts of chick embryo essence and trehalose, and freeze-drying. The Bifidobacterium longum cell lysate contains all intracellular substances and a part of cell wall lytic components of Bifidobacterium longum, is used in anti-aging and whitening cosmetics in combination with chick embryo essence, and has excellent use effects. The invention also provides anti-aging and whitening cosmetics containing the freeze-dried powder composition mentioned above.

Description

technical field [0001] The invention belongs to the technical field of cosmetics, and in particular relates to a composition freeze-dried powder containing bifidobacterium longum cell lysate, a preparation method thereof and an application in cosmetics. Background technique [0002] The bifidobacterium longum ferment lysate contains bifidobacterium longum ( Bifidobacterium longum ) production of essential components and metabolites of long dichotomy (active proteins, peptides and cytokines), cytoplasmic components, cell wall components and polysaccharide complexes. These substances can specifically activate and support the skin's own protection and repair mechanisms to fight against premature skin aging. It is used in cosmetics to promote skin anti-ultraviolet, repair and rebuild perfect structure, anti-aging, moisturizing protection and solve sensitive and problem skin. Their effects on problem skin mainly include: 1) Activation of normal DNA repair mechanism after UV irra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/98A61Q19/02A61Q19/08A61Q19/10
Inventor 黄勇前王维荣吴宏忠谢承光曹平生唐娟梁文娟
Owner SHANGHAI DELTA IND