Application of yeast cultures in promoting proliferation of cellulose decomposition bacteria

A technology of cellulolytic bacteria and yeast culture, applied in the direction of microorganism-based methods, bacteria, fermentation, etc., can solve the problems of metabolite composition and concentration differences

Inactive Publication Date: 2015-04-22
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even when the same yeast strain is used, different medium compositions or different fermentation production control processes can lead to significant differences in the composition and concentration of metabolites in yeast culture products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, yeast culture and its preparation method and application

[0039] 1. Preparation of Yeast Culture

[0040] 1. Preparation of culture medium

[0041] Liquid seed medium: weigh 10g glucose, 5g yeast powder, 5g peptone and 0.1g MnSO 4 ·H 2 O, after heating and dissolving with 1L of distilled water, sterilize at 121°C and 0.1Mpa for 15min, and set aside.

[0042] Liquid fermentation medium: weigh 10g glucose, 10g sucrose, 10g molasses, 10g peptone, 10g yeast extract, 5g ammonium sulfate, 0.1g KH 2 PO 4 , 0.1g MgSO 4 ·7H 2 O, 0.1g MnSO 4 ·H 2 O, 0.1g CaCl 2 , 0.1gCuSO 4 ·5H 2 O, 0.1g ZnSO 4 ·7H 2 O and 0.1 g FeSO 4 4H 2 O, after dissolving in distilled water, adjust the pH value to 4.0 with 0.1mol / L HCl solution, dilute to 1L, sterilize at 121°C, 0.1Mpa for 15min, and set aside.

[0043] 2. Obtaining of seed solution

[0044] The freeze-dried and preserved Saccharomyces cerevisiae (Saccharomyces cerevisiae CGMCC No.2.1882) was activated for thr...

Embodiment 2

[0061] Embodiment 2, yeast culture and its preparation method and application

[0062] 1. Preparation of Yeast Culture

[0063] 1. Preparation of culture medium

[0064] Liquid seed medium: weigh 40g glucose, 40g yeast powder, 40g peptone and 4.0g MnSO 4 ·H 2 O, after heating and dissolving with 1L of distilled water, sterilize at 121°C and 0.1Mpa for 15min, and set aside.

[0065] Liquid fermentation medium: 100g glucose, 100g sucrose, 100g molasses, 300g peptone, 300g yeast extract, 40g ammonium sulfate, 10g KH 2 PO 4 , 10g MgSO 4 ·7H 2 O, 4.0g MnSO 4 ·H 2 O, 4.0 g CaCl 2 , 4.0g CuSO 4 ·5H 2 O, 4.0g ZnSO 4 ·7H 2 O and 4.0 g FeSO 4 4H 2 O, after dissolving in distilled water, adjust the pH value to 6.5 with 0.1mol / L NaOH solution, set the volume to 1L, sterilize at 121°C and 0.1Mpa for 15min, and set aside.

[0066] 2. Obtaining of seed solution

[0067] The freeze-dried and preserved Saccharomyces cerevisiae (Saccharomyces cerevisiae CGMCC No.2.1882) was act...

Embodiment 3

[0078] Embodiment 3, yeast culture and its preparation method and application

[0079] 1. Preparation of Yeast Culture

[0080] 1. Preparation of culture medium

[0081] Liquid seed medium: 20g glucose, 20g yeast powder, 20g peptone and 2.0g MnSO 4 ·H 2 O.

[0082] Liquid fermentation medium: 50g glucose, 50g sucrose, 50g molasses, 50g peptone, 50g yeast extract, 20g ammonium sulfate, 5.0g KH 2 PO 4 , 5.0 g MgSO 4 ·7H 2 O, 2.0g MnSO 4 ·H 2 O, 2.0g CaCl 2 , 2.0 g CuSO 4 ·5H 2 O, 2.0g ZnSO 4 ·7H 2 O and 2.0 g FeSO 4 4H 2O, after dissolving in distilled water, adjust the pH value to 5.5 with 0.1mol / L NaOH solution, set the volume to 1L, sterilize at 121°C and 0.1Mpa for 15min, and set aside.

[0083] 2. Obtaining of seed solution

[0084] The freeze-dried and preserved Saccharomyces cerevisiae (Saccharomyces cerevisiae CGMCC No.2.1882) was activated for three generations and then inserted into the above-mentioned liquid seed medium, and cultured with shaking at 3...

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Abstract

The invention discloses the application of a yeast culture in promoting proliferation of cellulose decomposition bacteria. The yeast culture is prepared by a method comprising the following steps: saccharomyces cerevisiae CGMCC No.2.1882 is subjected to liquid fermentation, then fermentation liquor is collected, the saccharomyces cerevisiae CGMCC No.2.1882 in the fermentation liquor is removed, then the obtained sterile liquid is the yeast culture. The experimental result shows that when the yeast culture obtained by the process is used for culturing the cellulose decomposition bacteria to produce bacteroides succinogenes, ruminococcus flavefaciens and ruminococcus albus, the biomass liveweights of the bacteroides succinogenes, the ruminococcus flavefaciens and the ruminococcus albus are respectively increased by 75.6 to 80.9 percent, 69.89 to 82.75 percent, and 64.78 to 76.76 percnet. The application disclosed by the invention provides an important way for raising productivity level of ruminant and optimizing feed composition.

Description

technical field [0001] The invention relates to the application of a yeast culture in promoting the proliferation of cellulolytic bacteria. Background technique [0002] At present, roughage such as corn stalks is still used as part of the energy source for ruminants in northern my country. Due to the rough texture and poor palatability of this type of feed, the feed intake of ruminants is reduced, which affects the absorption of nutrients and results in low production efficiency. Therefore, many researchers have conducted a lot of research on how to improve the digestibility of this type of roughage. Studying the utilization of roughage by ruminants is actually the process of studying how microorganisms in the rumen utilize crude fiber. The main cellulolytic bacteria in the rumen include Filamentobacterium succinogenes, Ruminococcus bovis and Ruminococcus albicans. In addition, there are some secondary cellulolytic bacteria, which also play a certain role in fiber degradat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/02C12R1/865C12R1/01
Inventor 刘萍解洛香徐乐
Owner CHINA AGRI UNIV
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