Application of yeast cultures in promoting proliferation of cellulose decomposition bacteria
A technology of cellulolytic bacteria and yeast culture, applied in the direction of microorganism-based methods, bacteria, fermentation, etc., can solve the problems of metabolite composition and concentration differences
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Embodiment 1
[0038] Embodiment 1, yeast culture and its preparation method and application
[0039] 1. Preparation of Yeast Culture
[0040] 1. Preparation of culture medium
[0041] Liquid seed medium: weigh 10g glucose, 5g yeast powder, 5g peptone and 0.1g MnSO 4 ·H 2 O, after heating and dissolving with 1L of distilled water, sterilize at 121°C and 0.1Mpa for 15min, and set aside.
[0042] Liquid fermentation medium: weigh 10g glucose, 10g sucrose, 10g molasses, 10g peptone, 10g yeast extract, 5g ammonium sulfate, 0.1g KH 2 PO 4 , 0.1g MgSO 4 ·7H 2 O, 0.1g MnSO 4 ·H 2 O, 0.1g CaCl 2 , 0.1gCuSO 4 ·5H 2 O, 0.1g ZnSO 4 ·7H 2 O and 0.1 g FeSO 4 4H 2 O, after dissolving in distilled water, adjust the pH value to 4.0 with 0.1mol / L HCl solution, dilute to 1L, sterilize at 121°C, 0.1Mpa for 15min, and set aside.
[0043] 2. Obtaining of seed solution
[0044] The freeze-dried and preserved Saccharomyces cerevisiae (Saccharomyces cerevisiae CGMCC No.2.1882) was activated for thr...
Embodiment 2
[0061] Embodiment 2, yeast culture and its preparation method and application
[0062] 1. Preparation of Yeast Culture
[0063] 1. Preparation of culture medium
[0064] Liquid seed medium: weigh 40g glucose, 40g yeast powder, 40g peptone and 4.0g MnSO 4 ·H 2 O, after heating and dissolving with 1L of distilled water, sterilize at 121°C and 0.1Mpa for 15min, and set aside.
[0065] Liquid fermentation medium: 100g glucose, 100g sucrose, 100g molasses, 300g peptone, 300g yeast extract, 40g ammonium sulfate, 10g KH 2 PO 4 , 10g MgSO 4 ·7H 2 O, 4.0g MnSO 4 ·H 2 O, 4.0 g CaCl 2 , 4.0g CuSO 4 ·5H 2 O, 4.0g ZnSO 4 ·7H 2 O and 4.0 g FeSO 4 4H 2 O, after dissolving in distilled water, adjust the pH value to 6.5 with 0.1mol / L NaOH solution, set the volume to 1L, sterilize at 121°C and 0.1Mpa for 15min, and set aside.
[0066] 2. Obtaining of seed solution
[0067] The freeze-dried and preserved Saccharomyces cerevisiae (Saccharomyces cerevisiae CGMCC No.2.1882) was act...
Embodiment 3
[0078] Embodiment 3, yeast culture and its preparation method and application
[0079] 1. Preparation of Yeast Culture
[0080] 1. Preparation of culture medium
[0081] Liquid seed medium: 20g glucose, 20g yeast powder, 20g peptone and 2.0g MnSO 4 ·H 2 O.
[0082] Liquid fermentation medium: 50g glucose, 50g sucrose, 50g molasses, 50g peptone, 50g yeast extract, 20g ammonium sulfate, 5.0g KH 2 PO 4 , 5.0 g MgSO 4 ·7H 2 O, 2.0g MnSO 4 ·H 2 O, 2.0g CaCl 2 , 2.0 g CuSO 4 ·5H 2 O, 2.0g ZnSO 4 ·7H 2 O and 2.0 g FeSO 4 4H 2O, after dissolving in distilled water, adjust the pH value to 5.5 with 0.1mol / L NaOH solution, set the volume to 1L, sterilize at 121°C and 0.1Mpa for 15min, and set aside.
[0083] 2. Obtaining of seed solution
[0084] The freeze-dried and preserved Saccharomyces cerevisiae (Saccharomyces cerevisiae CGMCC No.2.1882) was activated for three generations and then inserted into the above-mentioned liquid seed medium, and cultured with shaking at 3...
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