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Method for producing anti-stress growth promoting fodder additive

A feed additive and production method technology, applied in animal feed, animal feed, application, etc., can solve problems that have not been reported, and achieve the effects of inhibiting the occurrence of hazards, low production costs, and promoting growth

Active Publication Date: 2012-04-25
佛山播恩生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In the past, yeast fermentation cultures have been used as feed additives in a variety of products on the market, but Armillaria fermented products are used as feed additives or the fermentation products of the co-fermentation of the above-mentioned Armillaria and Saccharomyces cerevisiae are used as feed additives and The preparation method has not been reported

Method used

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  • Method for producing anti-stress growth promoting fodder additive

Examples

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Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: (a kind of production method 1 of anti-stress growth-promoting feed additive)

[0020] Follow these steps:

[0021] (1) Seed liquid culture of Saccharomyces cercvisiae strain 2.1952: insert the original slant strain of Saccharomyces cerevisiae into the test tube seed medium, and cultivate it statically at 26-28°C for 30 hours to become test tube seeds; Put the seeds in the test tube into the seed medium of the Erlenmeyer flask, and after static cultivation at 26-28°C for 24 hours, they will become seeds of the flask; Static culture for 20h to become Saccharomyces cerevisiae fermented seed liquid, for subsequent use; the seed culture medium of above-mentioned test tube, Erlenmeyer flask and Karl Fischer tank all adopts 10 ° of Bx saccharified wort culture medium;

[0022] The preparation method of the 10°Bx saccharification wort culture medium: take a certain amount of barley malt, crush it, add 60°C water 4 times the weight of the malt, keep saccharificat...

Embodiment 2

[0026] Embodiment 2. (a kind of production method 2 of anti-stress growth-promoting feed additive)

[0027] In this example, the seed liquid culture of step (1) Saccharomyces cerevisiae: After the inoculated test tube seed medium was cultured statically at 26-28°C for 27 hours; After static culture at 28°C for 22 hours; insert 10% of the inoculum into the seed culture medium of the Karl Fischer jar, and cultivate Saccharomyces cerevisiae fermented seed liquid through static culture at 26~28°C for 18 hours; step (2) seed liquid cultivation of Armillaria armillaria: After the inoculated Erlenmeyer flask culture medium was cultured on a shaker at 26-28°C and 180-200rpm for 6.5 days; it was inserted into the medium of the first-level seed tank according to the inoculum amount of 8%, and was ventilated at 26-28°C and 180-200rpm. The amount is 1:0.5 after culturing for 60 hours; access the culture medium of the secondary seed tank according to 10% inoculum amount, and cultivate it f...

Embodiment 3

[0028] Embodiment 3. (a kind of production method 3 of anti-stress growth-promoting feed additive)

[0029] In this example, step (1) Saccharomyces cerevisiae seed liquid culture: After the inoculated test tube seed medium was cultured statically at 26-28°C for 24 hours; After static culture for 20 hours; insert 10% inoculum into the seed culture medium of the Karst jar, and statically cultivate at 26-28°C for 16 hours to form a fermented seed liquid of Saccharomyces cerevisiae; After the culture medium is cultured on a shaker at 26-28°C and 180-200rpm for 6.0 days; the inoculation amount is 5% into the medium of the first-level seed tank, and the culture medium is 26-28°C, 180-200rpm, and the ventilation rate is 1:0.6 After culturing for 48 hours under the same conditions; 5% inoculation amount was inserted into the medium of the secondary seed tank, and cultivated under the same conditions for 72 hours to form Armillaria fermented seed liquid; the composition of the medium w...

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Abstract

The invention discloses a method for producing anti-stress growth promoting fodder additive, and belongs to the technical field of microbial fermentation engineering. The method comprises the following processing steps: (1) culturing saccharomyces cerevisiae seed liquid, (2) culturing honey fungus seed liquid, (3) preparing saccharomyces cerevisiae and honey fungus mixed fermentation production solution, and (4) performing adsorption treatment on the mixed fermentation production solution, packing and the like. In the method, cheap and available agricultural and sideline products such as beanmeal, corn meal and the like are used as main raw materials; the fodder additive is produced by adopting a strain liquid mixed fermentation process; and the fodder additive has the effects of low production cost, good effect, safety, no residue, stress resistance, growth promotion and the like. The fodder additive can be popularized and applied in livestock and poultry breeding industry and fodder production enterprises.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation engineering, and in particular relates to a method for producing an anti-stress and growth-promoting feed additive by mixed fermentation of Armillaria armillaria and Saccharomyces cerevisiae. technical background [0002] The so-called stress (Stress) is the non-specific response of the animal body to various external or internal abnormal stimuli. Livestock and poultry are subjected to various and considerable stresses throughout the production process, including: sudden changes in the surrounding environment, sudden changes in diets, toxic ingredients in feed (such as mycotoxins), environmental Changes in temperature (high temperature, cold) and humidity (humidity), etc., or the use of various preventive medicines and vaccinations to prevent the spread of infectious diseases are important sources of stress. It is generally believed that stress is completed through the nervous syst...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/16A23K10/18
Inventor 李孝辉许尧兴皮雄娥许少春叶琪明李艳丽
Owner 佛山播恩生物科技有限公司
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