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Duck flavivirus, and vaccine and kit thereof

A duck flavivirus and kit technology, applied in the direction of antiviral agents, antiviral immunoglobulins, viral peptides, etc., can solve the problems of limited means of treatment and prevention of laying ducks, cessation, and abandonment of laying ducks.

Active Publication Date: 2012-07-04
SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of duck yellow virus and its vaccine, kit, described this duck yellow virus and its vaccine, kit will solve the problem that in the prior art there is no appetite for laying ducks, egg production drops or even Cessation, a technical problem with limited means of treatment and prevention of the phenomenon leading to partial mortality of laying ducks

Method used

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  • Duck flavivirus, and vaccine and kit thereof
  • Duck flavivirus, and vaccine and kit thereof
  • Duck flavivirus, and vaccine and kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Pathogen Isolation

[0052] Take the spleen tissue of the diseased duck, add 1ml PBS (containing 100 units of penicillin and 50ug of streptomycin) and grind it. After centrifugation, take the supernatant, sterilize with a 0.2um filter, and inoculate SPF chicken embryos. Discard the chicken embryos that died within 24 hours; collect the dead chicken embryos after 24 hours, thickening of the allantoic membrane and systemic hemorrhage of the embryo body can be seen (see figure 1 ); allantoic fluid, chicken embryo body and allantoic membrane were collected respectively.

Embodiment 2

[0053] The identification of embodiment 2 virus mainly exists position in chicken embryo

[0054] Take embryo body leachate and allantoic fluid and dilute to 10 times respectively -1 , 10 -2 , 10 -3 , 10 -4 , each titer 100ul inoculated three 9-day-old SPF chicken embryos through the allantoic cavity, placed them in an incubator at 37°C, and observed and recorded the death of the chicken embryos. It was found that the virus mainly existed in the embryo body of the chicken embryo and its allantoic membrane, and the virus content in the allantoic fluid was only one-tenth of that in the above-mentioned parts.

Embodiment 3

[0055] Example 3 Virus Amplification

[0056] After grinding the collected embryo body and its allantoic membrane, it was frozen and thawed three times and then centrifuged to get the supernatant, which was diluted ten times with sterilized PBS and inoculated into 40 9-day-old SPF chicken embryos through the allantoic cavity, and incubated at 37°C Incubation in the box. The chicken embryos that died within 24 hours were discarded; 48-120 hours after inoculation, all the chicken embryos died; the chicken embryo body, allantoic membrane and allantoic fluid were collected respectively.

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Abstract

The invention belongs to the field of bioengineering and discloses duck flavivirus. The amino acid sequence of an envelope protein E is shown as SEQ ID NO:4 or the homology of the amino acid sequence which is shown as the SEQ ID NO:4 is over 98 percent. The invention also discloses vaccine for preventing duck flavivirus, and a kit for detecting duck flavivirus; a new virus of duck flavivirus disease which causes output reduction and death of laying duck are separated and identified by various detection means, a method for diagnosing duck flavivirus disease is established, the vaccine for duck flavivirus disease is developed, important basis and means are provided for preventing duck flavivirus, and relative duck flavivirus diseases which cause the output reduction and death of laying duck can be prevented and diagnosed by using the vaccine and a diagnostic reagent which are developed by using the virus.

Description

Technical field: [0001] The invention belongs to the field of bioengineering, and in particular relates to a virus, specifically a duck yellow virus and its vaccine and kit. Background technique: [0002] China is a big poultry country, and the poultry industry is developing rapidly. The annual breeding volume of poultry has reached 12 billion, ranking first in the world. The proportion of the economy of the poultry industry in the national economy is gradually increasing, and its economy accounts for a large part of farmers' income. It can be seen that the health status of poultry plays a decisive role in the development of my country's national economy and social stability. In recent years, my country's waterfowl breeding industry has been growing steadily. According to the statistics of the Food and Agriculture Organization of the United Nations (FAO), the total annual breeding of ducks and geese in my country has reached nearly 5 billion feathers, and the annual output...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12N15/40C12N15/63C07K14/18C07K16/10C12Q1/70C12Q1/68G01N33/569A61K39/12A61K48/00A61P31/14C12R1/93
Inventor 李泽君颜丕熙闫丽萍滕巧泱
Owner SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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