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Preparation method for urine monohydroxyphenyl metabolites detection reagent

A technology of monohydric phenols and detection reagents, which is applied in the field of preparation of urine monohydric phenols metabolite detection reagents, can solve the problems of no discovery, achieve low false positive rate and false negative rate, high stability and sensitivity , the effect of low production cost

Inactive Publication Date: 2013-10-30
宜春中奇金域生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

By detecting the content of monohydric phenol metabolites in urine, it can be used to judge the possibility of malignant tumors in advance, and the discovery of preclinical malignant tumors is of great significance for the reversal and cure of tumor cells. Research

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 : Prepare 1750ml urine monohydric phenol metabolite detection reagent

[0018] This reagent is composed of three kinds of solutions, according to the volume ratio, that is, A solution: B solution: C solution = 1:2:1, respectively prepared as 250ml, 500ml and 250ml, of which

[0019] 1, A solution: take 64g mercury, be dissolved in the nitric acid 96ml of specific gravity 1.42g / ml, treat that mercury dissolves completely, add the distilled water of 166.7ml, promptly be mixed with the A solution of 250ml;

[0020] 2. Solution B: the concentration of nickel nitrate solution is 0.1g / ml, weigh 80g of nickel nitrate 6 water, dissolve it in an appropriate amount of 5mol / L nitric acid solution, then add 5mol / L nitric acid solution to constant volume to 500ml, that is Prepare a nickel nitrate solution with a concentration of 0.1g / ml;

[0021] 3. Solution C: the concentration of mercuric sulfate solution is 0.1g / ml, weigh 25g of mercuric sulfate, dissolve it in an app...

Embodiment 2

[0023] Example 2 : Prepare 1750ml urine monohydric phenol metabolite detection reagent

[0024] This reagent is composed of three kinds of solutions, according to the volume ratio, that is, A solution: B solution: C solution = 1:2:1, respectively prepared as 250ml, 500ml and 250ml, of which

[0025] 1, A solution: with embodiment 1;

[0026] 2. Solution B: the concentration of nickel nitrate solution is 0.15g / ml, weigh 119.3g of nickel nitrate 6 water, dissolve it in an appropriate amount of 5mol / L nitric acid solution, then add 5mol / L nitric acid solution to constant volume to 500ml, that is Prepare a nickel nitrate solution with a concentration of 0.15g / ml.

[0027] 3, C solution: with embodiment 1.

[0028] Then, mix the above three solutions evenly, and finally, add 750ml of distilled water equal to the volume of the two solutions of A and B, stir evenly, and then prepare 1750ml of this reagent.

Embodiment 3

[0029] Example 3 : Prepare 1500ml urine monohydric phenolic metabolite detection reagent

[0030] This reagent is composed of three kinds of solutions, according to the volume ratio, that is, A solution: B solution: C solution = 1:1.5:1, respectively prepared as 250ml, 375ml and 250ml, of which

[0031] 1, A solution: with embodiment 1;

[0032] 2. Solution B: the concentration of nickel nitrate solution is 0.1g / ml, weigh 59.7g of nickel nitrate 6 water, dissolve it in an appropriate amount of 5mol / L nitric acid solution, then add 5mol / L nitric acid solution to dilute to 375ml, that is Prepare a nickel nitrate solution with a concentration of 0.1g / ml;

[0033] 3, C solution: with embodiment 1;

[0034] Then, mix the above three solutions evenly, and finally, add 625ml of distilled water equal to the volume of the two solutions A and B, stir evenly, and then prepare 1500ml of this reagent.

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Abstract

A preparation method for a urine monohydroxyphenyl metabolites detection reagent comprises the steps of preparing solution A, preparing solution B, preparing solution C, uniformly mixing solution A, solution B and solution C at the volume ratio of 1:(1.5-2):(0.5-1.5), adding distilled water into the mixture, and stirring the mixture uniformly, wherein solution A is a mixture prepared through reacting 1 part by weight of metallic mercury with 2-2.2 parts by weight of nitric acid and being diluted by distilled water; solution B is a nickel nitrate solution with the concentration of 0.05g / ml-0.15g / ml; solution C is a mercuric sulfate solution with the concentration of 0.05g / ml-0.15g / ml. The process for preparing testing reagents of monohydroxyphenyl metabolites in urine has the advantages of being low in production cost, and the prepared testing reagents of monohydroxyphenyl metabolites in urine is high in stability and sensitivity, low in false positive rate and false negative rate, and suitable for mass screening and clinical examination.

Description

technical field [0001] The invention relates to a preparation method of a urine monohydric phenol metabolite detection reagent. Background technique [0002] A significant increase in the level of urinary monohydric phenolic metabolites can indicate the occurrence of malignant disease. By detecting the content of monohydric phenol metabolites in urine, it can be used to judge the possibility of malignant tumors in advance, and the discovery of preclinical malignant tumors is of great significance for the reversal and cure of tumor cells. Research. Contents of the invention [0003] The purpose of the present invention is to provide a preparation method of urine monohydric phenolic metabolite detection reagent with low production cost. [0004] The preparation method of the urine monohydric phenol metabolite detection reagent of the present invention comprises the following steps: [0005] A solution preparation: use 1 part by weight of metallic mercury and 2 to 2.2 part...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/82
Inventor 陶海南王奇
Owner 宜春中奇金域生物科技股份有限公司
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