Method for identifying herbicide resistance of cotton leaves by cotton ball positioning

Abstract

The invention relates to plant physiology and pesticide science, in particular to a method for identifying herbicide resistance of cotton leaves by cotton ball positioning, which includes the steps: selecting material leaves for treatment, namely selecting intact functional leaves or leaves adjacent to those; secondly, cotton ball positioning, namely setting action spots on the front face of every functional leaf of each cotton plant clockwise or anticlockwise from a stipe, dipping cotton balls approximately in the same size with herbicide solutions having set concentrations, and sequentially placing the cotton balls dipped with the solutions having different concentrations at the corresponding action spots on the surface of each leaf; thirdly, observing action conditions of the treated spots to determine resistance levels of the spots. By the method, materials can be saved greatly, experimental scale can be reduced, artificial errors can be reduced greatly, results are precise and reliable, precision identification of the material resistance levels is technically guaranteed, and deep resistance research on current-generation material single plants is guaranteed. The method is quite simple in operation and easy to master.

Description

technical field [0001] The invention relates to plant physiology and pesticide science, in particular, the invention relates to a method for identifying herbicide resistance by locating cotton balls on cotton leaves. Background technique [0002] At present, the identification of cotton herbicide resistance is often carried out by seed germination identification method, different concentration herbicide spraying method in different periods, leaf spot coating growth point method at bud stage, cell line and other culture herbicide treatment methods, etc., but the seed germination identification method The amount of seeds is required to be large enough, but the method of spraying herbicides with different concentrations in different periods requires a large amount of seedlings in each period, and experimental errors will also be caused by uneven spraying and liquid drift. Points cannot represent the growth level of the entire cotton plant, and higher concentration treatment wil...

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Problems solved by technology

[0002] At present, the identification of cotton herbicide resistance is often carried out by seed germination identification method, different concentration herbicide spraying method in different periods, leaf spot coating growth point method at bud stage, cell line and other culture herbicide treatment methods, etc., but the seed germination identification method The amount of seeds is required to be large enough, but the method of spraying herbicides with different concentrations in different periods requires a large amount of seedlings in each period, and experimental errors will also be caused by uneven spraying and liquid drift. Points cannot represent the growth level of the entire cotton plant, and higher concentration treatment will cause the growth point of the cotton plant to be controlled and severely deformed, and the cotton plant will not be able to flower and set fruit, and all the above methods may kill the material. When a large number of materials are identified, the experimental scale is required to be large, which is not easy to realize

[0003] At the same time, when identifying limited or rare cotton materials for herbicide resistance, due to the limitation of the amount of materials, it is very likely to destroy the materials according to the above methods, and it is impossible to ensure that the resistance level or lethal concentration of the materials will be found

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