Method for preparing acellular bone
A decellularized bone and decellularized technology, which is applied in the field of preparing decellularized bone, can solve the problems of incomplete decellularization, bone matrix damage, strong rejection, etc., and achieve strong protective effect, short degreasing time, and avoid bone swelling.
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Embodiment 1
[0014] A method for preparing decellularized bone of the present invention, the decellularized bone is prepared from pig bone, bovine bone or sheep bone, comprising the following steps:
[0015] a. Process for preparing heterogeneous bone: cutting animal bone into 0.2cm flaky bone slices, removing surface grease;
[0016] B, virus inactivation process: the virus inactivation solution that the peracetic acid of mass concentration 0.1% and the sodium chloride of mass concentration 1% is used to carry out virus inactivation 0.5 hour with flake bone slice, virus inactivation is carried out with flake bone slice The mass ratio of live solution is 1:3;
[0017] c. Degreasing process: use a mixed degreasing agent with a mass concentration of 3.0% soda ash, a mass concentration of 3.0% caustic soda and a mass concentration of 0.5% Pingpingjia for degreasing for 1.0 hours, and the mass ratio of the flaky bone pieces to the mixed degreasing agent is 1:3;
[0018] d. Decellularizati...
Embodiment 2
[0022] A method for preparing decellularized bone of the present invention, the decellularized bone is prepared from pig bone, bovine bone or sheep bone, comprising the following steps:
[0023] a. Process for preparing heterogeneous bone: cutting animal bone into 0.3cm flaky bone slices, removing surface grease;
[0024] B, virus inactivation process: carry out virus inactivation 0.8 hour with the virus inactivation solution that the peracetic acid of mass concentration 1% and the sodium chloride of mass concentration 3% are made up of flake bone slice, and flake bone slice and virus inactivation The mass ratio of live solution is 1:4;
[0025] c, degreasing process: adopt the mixed degreasing agent that mass concentration 4.0% soda ash, mass concentration 4.0% caustic soda and mass concentration 0.8% sodium dodecylaminopropionate are degreasing 3.0 hours, the quality of flaky bone piece and mixed degreasing agent The ratio is 1:4;
[0026] d. Decellularization process:...
Embodiment 3
[0030] A method for preparing decellularized bone of the present invention, the decellularized bone is prepared from pig bone, bovine bone or sheep bone, comprising the following steps:
[0031] a. Process for preparing heterogeneous bone: cutting animal bone into 0.4cm flaky bone slices, removing surface grease;
[0032] B, virus inactivation process: carry out virus inactivation 1.0 hour with the virus inactivation solution that the peracetic acid of mass concentration 2% and the sodium chloride of mass concentration 5% are made up of flaky bone piece, flaky bone piece and virus inactivation The mass ratio of live solution is 1: 5;
[0033] C, degreasing process: the mixed degreasing agent that adopts mass concentration 5.0% soda ash, mass concentration 5.0% caustic soda and mass concentration 1.0% fatty alcohol polyoxyethylene ether to carry out degreasing 5.0 hours, the mass ratio of flaky bone piece and mixed degreasing agent is 1:5;
[0034] d. Decellularization pr...
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