Method for preparing acellular bone

A decellularized bone and decellularized technology, which is applied in the field of preparing decellularized bone, can solve the problems of incomplete decellularization, bone matrix damage, strong rejection, etc., and achieve strong protective effect, short degreasing time, and avoid bone swelling.

Active Publication Date: 2012-07-18
上海优越生物医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Clinically, the problems of strong antigenicity and strong rejection of xenograft bone transplantation have not been effectively resolved. The antigenicity of xenograft bone mainly comes from xenogeneic bone cells. Longer, lower efficiency; incomplete decellularization, easy to cause damage to bone matrix

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A method for preparing decellularized bone of the present invention, the decellularized bone is prepared from pig bone, bovine bone or sheep bone, comprising the following steps:

[0015] a. Process for preparing heterogeneous bone: cutting animal bone into 0.2cm flaky bone slices, removing surface grease;

[0016] B, virus inactivation process: the virus inactivation solution that the peracetic acid of mass concentration 0.1% and the sodium chloride of mass concentration 1% is used to carry out virus inactivation 0.5 hour with flake bone slice, virus inactivation is carried out with flake bone slice The mass ratio of live solution is 1:3;

[0017] c. Degreasing process: use a mixed degreasing agent with a mass concentration of 3.0% soda ash, a mass concentration of 3.0% caustic soda and a mass concentration of 0.5% Pingpingjia for degreasing for 1.0 hours, and the mass ratio of the flaky bone pieces to the mixed degreasing agent is 1:3;

[0018] d. Decellularizati...

Embodiment 2

[0022] A method for preparing decellularized bone of the present invention, the decellularized bone is prepared from pig bone, bovine bone or sheep bone, comprising the following steps:

[0023] a. Process for preparing heterogeneous bone: cutting animal bone into 0.3cm flaky bone slices, removing surface grease;

[0024] B, virus inactivation process: carry out virus inactivation 0.8 hour with the virus inactivation solution that the peracetic acid of mass concentration 1% and the sodium chloride of mass concentration 3% are made up of flake bone slice, and flake bone slice and virus inactivation The mass ratio of live solution is 1:4;

[0025] c, degreasing process: adopt the mixed degreasing agent that mass concentration 4.0% soda ash, mass concentration 4.0% caustic soda and mass concentration 0.8% sodium dodecylaminopropionate are degreasing 3.0 hours, the quality of flaky bone piece and mixed degreasing agent The ratio is 1:4;

[0026] d. Decellularization process:...

Embodiment 3

[0030] A method for preparing decellularized bone of the present invention, the decellularized bone is prepared from pig bone, bovine bone or sheep bone, comprising the following steps:

[0031] a. Process for preparing heterogeneous bone: cutting animal bone into 0.4cm flaky bone slices, removing surface grease;

[0032] B, virus inactivation process: carry out virus inactivation 1.0 hour with the virus inactivation solution that the peracetic acid of mass concentration 2% and the sodium chloride of mass concentration 5% are made up of flaky bone piece, flaky bone piece and virus inactivation The mass ratio of live solution is 1: 5;

[0033] C, degreasing process: the mixed degreasing agent that adopts mass concentration 5.0% soda ash, mass concentration 5.0% caustic soda and mass concentration 1.0% fatty alcohol polyoxyethylene ether to carry out degreasing 5.0 hours, the mass ratio of flaky bone piece and mixed degreasing agent is 1:5;

[0034] d. Decellularization pr...

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PUM

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Abstract

The invention discloses a method for preparing acellular bone, comprising the following step of processing animal bones into acellular bones by using processes of preparation of bone pieces, viral inactivation, degreasing, decellularization, freeze-drying, cutting, molding, packaging and irradiation disinfection. The method for preparing acellular bone has the advantages of short degreasing time, high efficiency, strong protection effect on bone tissue structures, complete decellularization and small damage to natural structures of bone extracellular matrixes.

Description

technical field [0001] The invention relates to a method for preparing decellularized bone. Background technique [0002] Bone repair has always been a clinical problem, and the current methods of treating bone defects are still based on autologous and allogeneic bone transplantation. Clinically, the problems of strong antigenicity and strong rejection of xenograft bone transplantation have not been effectively resolved. The antigenicity of xenograft bone mainly comes from xenogeneic bone cells. Long, low efficiency; incomplete decellularization, easy to cause damage to the bone matrix. Therefore, it is necessary to provide a new technical solution to solve the above problems. Contents of the invention [0003] The purpose of the present invention is to provide a method for preparing decellularized bone with shorter period, higher efficiency, complete decellularization and less damage to the natural structure of bone extracellular matrix. [0004] The technical scheme a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36
Inventor 潘华倩潘银根
Owner 上海优越生物医学科技有限公司
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