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MicroRNA (ribonucleic acid) specific expression profile and application thereof

A technology of specific expression profile and differential expression, applied in the field of MicroRNA specific expression profile and its application, tumor biomarkers and its clinical application, can solve the problems of tumor recurrence, one-sided prediction, etc., achieve economical rationality, promote reform, reduce The effect of treatment and medical costs

Inactive Publication Date: 2012-07-18
SHANGHAI FIRST PEOPLES HOSPITAL
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, we don't know why a certain proportion of patients still have tumor recurrence after surgery under the strict selection criteria of liver transplantation.
Looking at the current main liver transplantation admission criteria for liver cancer, they all focus on the gross morphological indicators of the tumor, and rarely involve laboratory examination indicators such as genes and protein molecules, which inevitably makes the evaluation of tumor biological behavior and prediction of prognosis one-sided. change

Method used

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  • MicroRNA (ribonucleic acid) specific expression profile and application thereof
  • MicroRNA (ribonucleic acid) specific expression profile and application thereof
  • MicroRNA (ribonucleic acid) specific expression profile and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Specimen preparation

[0038] The paraffin-embedded specimens of isolated liver tumors after liver cancer liver transplantation were respectively the tumor recurrence group after transplantation (5 cases) and the non-recurring tumor group (5 cases) after transplantation. 20 serial sections with a thickness of 10μm were installed in clean The EP tube is ready for use at room temperature.

[0039] 2. RNA extraction

[0040] 1. Sample processing

[0041] The above sample is ground into fine powder with liquid nitrogen, and finally transferred to a centrifuge tube with 1ml TRIzol, and shaken repeatedly for about 5 minutes. (If it is a cell sample, take about 106-107 cells into 1ml TRIzol, and pipet repeatedly to lyse.)

[0042] 2. Phase separation

[0043] After standing at room temperature for about 10 minutes, add 200μl of chloroform to each 1ml of TRIzol, close the lid, shake vigorously for about 1min, let stand at room temperature for 2~5min, then centrifuge at 12000g for 15m...

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Abstract

The invention belongs to the fields of biological and medical examination, and relates to microRNA (ribonucleic acid) specific expression profile and clinical application thereof. Tumor recurrence after liver transplantation for liver cancer can be judged by five miRNAs or comprehensive indexes of the microRNA specific expression profile, accuracy rate reaches 77%, further, tumor recurrence and prognosis conditions of an integral case group can be judged, tumor recurrence and prognosis conditions can also be judged for patients after Milan delamination of the patients by the aid of the 5 miRNAs or the comprehensive indexes, accordingly, the microRNA specific expression profile can be used for screening patients with high tumor recurrence risks after clinical liver transplantation for liver cancer, assists in judging prognosis and realizing early diagnosis of recurrence, and provides a basis for early intervention treatment and implementation of individualized treatment.

Description

Technical field [0001] The present invention belongs to the field of biology and medical testing, and relates to a tumor biomarker and its clinical application, in particular to a MicroRNA specific expression profile and its application. The MicroRNA specific expression profile is related to early tumor recurrence after liver transplantation of liver cancer Diagnosis is related to prognostic judgment. Background technique [0002] Since 2004, with the continuous progress in the research of tumor molecular markers, microRNA has become a hot spot in the research of molecular markers. The prior art discloses that MicroRNAs (miRNAs) are single-stranded small RNAs of about 21-23 bases, which are made from a single-stranded RNA precursor with a hairpin structure of about 70-90 bases in size through Dicer enzyme. Produced after processing, different from siRNA (double-stranded) but closely related to siRNA. It is speculated that these small non-coding RNAs (miRNAs) are involved in reg...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 彭志海韩中博樊军卫
Owner SHANGHAI FIRST PEOPLES HOSPITAL
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