Deer antler capillary electrophoresis DNA (deoxyribonucleic acid) fingerprint spectrum and identification method

Abstract

The invention relates to the technical field of identification of traditional Chinese medicines and specifically relates to a deer antler capillary electrophoresis DNA (deoxyribonucleic acid) fingerprint spectrum and an identification method. The deer antler capillary electrophoresis DNA fingerprint spectrum is finally constructed by extracting DNA, performing PCR (polymerase chain reaction) amplification and identifying PCR products; and the deer antler DNA fingerprint identification method comprises the steps of establishing a deer antler DNA standard fingerprint spectrum, establishing a sample DNA fingerprint spectrum and utilizing similarity software to perform result identification. The fingerprint spectrum identification method can be used for identifying deer antler samples. According to the identification method disclosed by the invention, authenticity identification can be performed on deer antler from the essence of inheritance, and the identification method has the advantages of simplicity, convenience, quickness, reliable results and the like.

Description

technical field [0001] The invention relates to identification technology of traditional Chinese medicinal materials, in particular to a deer antler capillary electrophoresis DNA fingerprint and an identification method. Background technique [0002] Deer antler is a traditional and precious traditional Chinese medicinal material. According to the "Chinese Pharmacopoeia" (2010 edition), its authentic product is the unossified young horns of the male deer Cervus nippon Temminck or the red deer Cervus elaphus Linnaeus. Essence and blood, strong bones and other effects. Due to its high price and high demand, counterfeit products often appear on the market. At present, velvet antler is mainly identified based on its external morphology, microscopic characteristics and physical and chemical properties. Although these methods can provide a basis for the identification and quality evaluation of velvet antler to a certain extent, they require rich identification experience and are...

AI Technical Summary

Problems solved by technology

[0005] 1. Poor repeatability and stability: Many factors will affect the amplification results of RAPD analysis, resulting in poor repeatability and stability of the analysis results

[0006] 2. Delayed detection technology: At present, the analysis of RAPD amplification products mainly adopts two methods: agarose gel electrophoresis and polyacrylamide gel electrophoresis

Polyacrylamide gel electrophoresis has high resolution and sensitivity, but the operation is cumbersome (including assembly, filling, electrophoresis, gel unloading, staining, etc.), time-consuming (the whole process takes hours to days), and the analysis efficiency is low. lower

Therefore, the current commonly used detection technology cannot meet the requirements of high resolution, high sensitivity, simplicity, and rapidity for the analysis of RAPD amplification products. The lack of suitable detection methods has seriously restricted the application of RAPD technology in the construction of species DNA fingerprints.

[0007] 3. The identification method is unreasonable: when using RAPD technology to identify species at present, it is usually to select some representative amplified bands in the electrophoresis pattern, and identify them according to the presence or absence of these bands

Although this method makes the result analysis easier, it reduces the polymorphism of the amplification product and increases the chance of false positive results

Moreover, artificially selecting some amplified bands and discarding other bands also reduces the objectivity of the analysis results.

In addition, this identification method can only reflect the presence or absence of amplified bands, and cannot reflect the difference in the strength of the amplified bands.

[0008] Due to the above reasons, the current reports on the construction of species DNA fingerprints and species identification using RAPD technology have only theoretical research significance and cannot be applied in practice.

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