Method for detecting biomolecule by using Fe3O4@Au nucleocapsid nano-probe

A technology of biomolecules and nanoprobes, applied in the direction of material excitation analysis, Raman scattering, etc., can solve the problems of low chemical stability and easy oxidation, achieve good chemical stability and biocompatibility, and improve efficiency , Simple and quick operation
CN102590174AInactive Publication Date: 2012-07-18XIAMEN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
XIAMEN UNIV
Publication Date
2012-07-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

A method for detecting a biomolecule by using a Fe3O4@Au nucleocapsid nano-probe relates to a surface-enhanced raman spectroscopy and includes the steps of first preparing a Fe3O4@Au probe; then preparing a gold probe; and finally raman-detecting. The method has the advantages of being capable of extracting, washing and concentrating a target biomolecule from a complex biological sample matrix conveniently by using an applied magnetic field, omitting multifarious operation such as centrifugation, filtering and the like, greatly improving detecting efficiency of the biomolecule, having good chemical stability and biological compatibility, and being capable of being used in detecting of the biomolecule safely. The probe combines the surface-enhanced raman spectroscopy, a detecting system has strong surface-enhanced raman signals, fast high-sensitivity detecting on the biomolecule is achieved by detecting enhanced raman signals of a marked molecule, and detecting sensitivity in an experiment is 10-7M. Compared with a traditional biological method, the method is simple and fast to operate, good in reproductively, and capable of achieving detecting of clinical samples.
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Description

technical field

[0001] The invention relates to a method based on surface-enhanced Raman spectroscopy, in particular to a method using Fe 3 o 4 Au core-shell nanoprobes for the rapid detection of biomolecules. Background technique

[0002] The rapid and accurate detection of biomolecules in biological samples has important research significance and practical value for obtaining chemical and biological information in life processes, diagnosing biomedical diseases, and chemical analysis. The detection of biomolecules includes the detection of organs, tissues, cells, and even DNA, proteins and other biomacromolecules. This requires that our detection devices must be gradually miniaturized, and at the same time ensure the high efficiency and sensitivity of detection, which is a great challenge faced by many traditional bioanalysis methods.

[0003] With the rapid development of nanoscience and technology, nanobiological probes composed of nanomaterials have become a new gener...

Claims

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