Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Construction method of seed melon HPLC (High Performance Liquid Chromatography) fingerprint spectrum and standard fingerprint spectrum thereof

A technology of standard fingerprints and fingerprints, which is applied in the construction of standard fingerprints and high-performance liquid chromatography (HPLC) fingerprints of seed melons, can solve the problems of unclear special active ingredients of seed melons, quality control problems in deep processing of seed melons, etc., and achieve detection Advanced means and strong operability

Active Publication Date: 2012-07-18
兰州大学科技园有限责任公司 +1
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, some special active ingredients of seed melon are not yet clear, which brings difficulties to the quality control of seed melon deep processing

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Construction method of seed melon HPLC (High Performance Liquid Chromatography) fingerprint spectrum and standard fingerprint spectrum thereof
  • Construction method of seed melon HPLC (High Performance Liquid Chromatography) fingerprint spectrum and standard fingerprint spectrum thereof
  • Construction method of seed melon HPLC (High Performance Liquid Chromatography) fingerprint spectrum and standard fingerprint spectrum thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Instruments and reagents: Agilent high performance liquid chromatography (Agilent 1200) and DAD secondary array detector were used; 10 batches of seed melons were produced in Jingtai, Jingyuan, Huining and other places in Gansu; methanol in the reagent was chromatographic Pure, acetic acid is analytically pure, and test water is deionized water;

[0029] (2) Preparation of the test solution: Remove the seeds from the melon, take out the pulp (0.5-1cm away from the rind) and pulverize it in a juice blender for 30 seconds, and filter the filtrate, which is the melon juice, and filter it with a 0.45μm filter membrane as The test solution;

[0030] (3) HPLC analysis: the chromatographic separation column packing is Sinochrom ODS-BP C18, 5 μm, 250 × 4.6mm; mobile phase is methanol and 0.2% acetic acid water; gradient elution method: 0min→2min→42min→45min, methanol: 2%→2%→70%→70%, injection volume 20 μL, detection wavelength 260 nm; column temperature 25°C; analyze the ...

Embodiment 2

[0034] The purpose of embodiment 2 is to compare the fingerprints of seed melons from different origins with the standard fingerprints of seed melons to prove the accuracy and practicability of the present invention.

[0035] Taking the seed melon from Jingtai-1 origin in Gansu as the test sample, Agilent high performance liquid chromatography (Agilent 1200) and DAD secondary array detector were used; the methanol in the reagent was chromatographically pure, the acetic acid was analytically pure, and the test water was deionized water; The following method makes the chromatogram of Gansu Jingtai-1 origin seed melon;

[0036] (1) Preparation of the test solution: remove the seeds from the melon, take out the pulp (0.5-1 cm from the melon rind) and pulverize it in a juice blender for 30 seconds, and filter the filtrate, which is the melon juice, and filter it with a 0.45 μm filter membrane to serve as The test solution;

[0037] (2) HPLC analysis: the chromatographic separation c...

Embodiment 3

[0046] The chromatograms of the seed melon juice used as raw materials for different batches of seed melon drinks were prepared and analyzed. The seed melon juice described in this embodiment is the seed melon that has been washed, deseeded, crushed, squeezed and filtered, enzyme-inactivated, sterilized, filled and stored under clean conditions with the addition of an appropriate amount of food preservatives, and used as a raw material for producing a seed melon beverage.

[0047] Use Agilent high performance liquid chromatography (Agilent 1200) and DAD secondary array detector; the methanol in the reagent is chromatographically pure, the acetic acid is analytically pure, and the test water is deionized water; according to the following method, the raw material of seed melon beverage—seed melon juice 1 - Chromatogram of batch 1;

[0048] (1) Preparation of the test solution: under clean conditions, the clarified solution of 1-1 batch samples of the seed melon beverage raw mate...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a construction method of a seed melon HPLC (High Performance Liquid Chromatography) fingerprint spectrum and a standard fingerprint spectrum thereof. A seed melon is deseeded, melon pulp is taken out and is arranged in a juice stirrer to be smashed for 30s, and filtrate which is seed melon juice is leached and filtered with a filter membrane of 0.45 mum, and then is used as a sample solution. HPLC analysis is carried out on the sample solution, the chromatographic column is SinochromODS-BPC18, 5mum, 250*4.6mm, the column temperature is 25 DEG C, the flowing phase is methanol and 0.2% of acetic acid water, a gradient elution way is adopted, the detection wavelength is 260 nm, and the seed melon HPLC fingerprint spectrum is obtained. 10 batches of seed melon HPLC fingerprint spectrums are compared to determine the common fingerprint characteristics and obtain the seed melon standard fingerprint spectrum. The standard fingerprint spectrum can be used for effectively monitoring the quality of seed melons and seed melon highly processed products.

Description

technical field [0001] The invention belongs to the technical field of natural plant analysis, and relates to a method for constructing natural plant fingerprints, in particular to a method for constructing high-performance liquid chromatography (HPLC) fingerprints of seed melons and standard fingerprints thereof. Background technique [0002] Fingerprint technology is currently internationally recognized as a practical and scientific quality control method for natural plants and their products. [0003] At present, various fingerprint control technology systems in line with the characteristics of natural medicines (plants) are being studied and established in the world. The US Food and Drug Administration (FDA) allows chromatographic fingerprints to be provided in the application materials for herbal health products; the World Health Organization (WHO) in In 1996, the guidelines for the evaluation of herbal medicines also stipulated that if the active ingredients of herbal ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 常蓬彬孙小明王勤孙丽枫
Owner 兰州大学科技园有限责任公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com