Stabilizer for measuring content of total sugar and reducing sugar by direct titration method and preparation method of stabilizer

Abstract

The invention discloses a stabilizer for measuring content of total sugar and reducing sugar by a direct titration method. The stabilizer is obtained by grinding and uniformly mixing 50 to 90 percent of a complexing masking agent, 5 to 25 percent of a surfactant and 5 to 25 percent of a polymer compound, wherein the complexing masking agent is bivalent metal salt. By the stabilizer, potassium ferrocyanide complexes are not increased at a reaction end point, deep yellow potassium ferrocyanide complexes are not continuously generated so as not to influence judgment on the reaction end point, so that the reaction end point is accurately judged. In addition, by use of the stabilizer, uniformly covered air bubbles can be generated in reaction solution in a boiling state, the reaction system is rapidly uniformly mixed in 0.5 to 1 second, and the reproducibility and precision of the titration are high. The comparison of the results of precision tests and accuracy tests shows that the precisions of two results are significantly different in case of adding the stabilizer or not, but the accuracy has no significant difference, namely, the stabilizer has an effect of greatly improving the precision of the direct titration method.

Description

technical field [0001] The invention belongs to the technical field of detection, in particular to the detection of total sugar and reducing sugar in food, in particular to a stabilizer for determining the content of total sugar and reducing sugar by direct titration, a preparation method and application thereof. Background technique [0002] The determination method of total sugar and reducing sugar in food is a kind of titration method, and the direct titration method (also known as potassium ferrocyanide titration method) is commonly used at present. [0003] The direct titration method is to titrate under boiling conditions. The principle is that glucose and copper hydroxide undergo oxidation-reduction reactions under boiling conditions to form cuprous oxide precipitates, and cuprous oxide and potassium ferrocyanide in the reaction system form coordination compounds. Titration At the end point, the excess glucose and the indicator methylene blue undergo a redox reaction,...

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Problems solved by technology

[0005] Aiming at the problem that the titration end point is difficult to accurately determine and the reaction uniformity is poor when the direct titration method is used to measure the content of total sugar and reducing sugar at present, the primary purpose of the present invention is to provide a stabilizer, which can make the system reach the titration end point. The reaction solution is fast and uniform, and the color of the reaction end point is always in a colorless state, which is convenient for confirming the end point and reducing titration errors

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