On-line sample preparation device for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)

An auxiliary laser and sample preparation technology, applied in the field of mass spectrometry-oriented sample preparation devices, can solve problems such as sample backmixing, zone broadening and cross-contamination, and achieve the effects of retaining resolution, good application prospects, and simplifying the sample preparation process.

Active Publication Date: 2012-08-01
XIAMEN CHROMATOGRAPHIC ANALYSIS INSTR CO LTD
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Problems solved by technology

[0005] The purpose of the present invention is to provide a simple and reliable method that is not only simple and reliable, but also can fully utilize the liquid

Method used

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  • On-line sample preparation device for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)
  • On-line sample preparation device for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)
  • On-line sample preparation device for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)

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Embodiment Construction

[0025] The following embodiments will further illustrate the present invention in conjunction with the accompanying drawings.

[0026] see figure 1 , the embodiment of the present invention is provided with a first-stage T-shaped orthogonal structure microfluidic chip, a second-stage T-shaped orthogonal structure microfluidic chip, a matrix reagent injection pump and a target plate 3 .

[0027] The first-stage T-shaped orthogonal structure microfluidic chip is used to prepare the sample separated by liquid chromatography into liquid droplets, and mix the aqueous phase eluent A of liquid chromatography with another immiscible oil Phase B introduces the microfluidic chip of the first-stage T-shaped orthogonal structure at the same time, and the inlet of the vertical arm 11 of the microfluidic chip of the first-stage T-shaped orthogonal structure is connected to the outlet of the aqueous phase eluent of the liquid chromatography; The left cross arm 121 of the first-stage T-shape...

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Abstract

The invention relates to a sample preparation device for mass spectrometry (MS), and provides an on-line sample preparation device for MALDI-MS. The device comprises two levels of T-shaped orthorhombic microfluidic chips, a matrix reagent injection pump and a target plate. The inlet of a vertical arm of a first level of the T-shaped orthorhombic microfluidic chip is connected with the outlet of an aqueous phase leacheate of a liquid chromatogram, the left cross arm is connected with an oil phase outlet, and the right cross arm is a sample liquid drop outlet. The inlet of a vertical arm of a second level of the T-shaped orthorhombic microfluidic chip is connected with the outlet of the matrix reagent injection pump, the left cross arm is connected with the sample liquid drop outlet of the first level of the T-shaped orthorhombic microfluidic chip, and the right cross arm is the outlet of collected liquid drop samples, namely target liquid drops. The target plate is arranged under the target liquid drop outlet. The on-line sample preparation device for the MALDI-MS is simple and reliable, and analysis of liquid chromatography-mass spectrometry can be worked efficiently.

Description

technical field [0001] The invention relates to a mass spectrometry-oriented sample preparation device, in particular to an on-line sample preparation device for matrix-assisted laser desorption ion source mass spectrometry which is used in conjunction with liquid chromatography separation technology. Background technique [0002] With the completion of the Human Genome Sequencing Project, proteomics research has gradually become the focus of life science research. The difficulty of proteomics research lies in the high complexity of its samples, which also determines that proteomics research strongly relies on high-resolution separation technology and identification technology with mass spectrometry as the core. [0003] The emergence of matrix-assisted laser desorption ionization technology and electrospray ionization technology makes it possible to analyze biological macromolecules by mass spectrometry. Electrospray mass spectrometry adopts the sampling technology of dire...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/72
Inventor 张博肖志良王雪
Owner XIAMEN CHROMATOGRAPHIC ANALYSIS INSTR CO LTD
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