Preparation method of relaxin wild type and mutant and relaxin wild type and mutant prepared by preparation method

Abstract

The invention discloses a method of preparing a recombinant human relaxin wild type and a mutant by using a genetic engineering means. The method comprises the following steps of: designing and synthesizing an amino acid codon gene sequence favored by yeast of the recombinant human relaxin wild type and the mutant; (2) designing an amino acid sequence of a human relaxin precursor of the recombinant wild type and the mutant; (3) constructing a secretion type recombinant yeast expression vector of the human relaxin wild type and the mutant; and (4) performing secretion expression of the recombinant wild type and the relaxin mutant. The invention discloses a method of expressing the human relaxin wild type and the mutant by using a genetic engineering means. According to the method, high-efficiency expression of the human relaxin mutant is realized through a pichia pastoris expression system by using a modified secretion type expression vector pPICZ. The method disclosed by the invention has the advantages of high yield, simplicity, convenience, easiness, low cost and the like. The recombinant human relaxin wild type and the mutant have effects of expanding blood vessels and can be used for treating cardiovascular diseases such as hypertension and heart failure, wound healing and treating expanded cervix caused by delivery.

Description

technical field [0001] The invention relates to human ovarian relaxin in the field of biomedicine, in particular to a preparation method and use of a relaxin compound with multiple uses or a wild type and a mutant. Background technique [0002] It is well known that mature human ovarian relaxin is an approximately 6000 Dalton peptide hormone that plays an important role in pregnancy hormone relaxation. H2 relaxin promotes the reproductive tract labor process, including cervical ripening, thickened endometrium, increased blood vessels during pregnancy, and has an effect on collagen synthesis. Human relaxin mainly acts on the remodeling of the reproductive tract before parturition, thereby promoting the labor process. During pregnancy and childbirth, it regulates the reorganization of proteins in the connective tissue of target organs to obtain the necessary changes in organ architecture. It also inhibits cervical ripening, including premature labor and childbirth. [0003]...

AI Technical Summary

Problems solved by technology

Human relaxin H1 and H2 show considerable nucleotide and amino acid sequence homology, however, there are also some significant sequence differences, especially in the amino-terminal regions of the A and B chains

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