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Soybean antigenic protein degradation strain and application thereof

A soybean antigen protein and strain technology, applied in the field of agricultural microorganisms, can solve the problems of piglet diarrhea, growth, obstruction, etc.

Inactive Publication Date: 2013-09-04
辽宁德宝农牧集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that soybean antigenic protein in soybean meal can cause diarrhea and growth retardation in piglets, and to provide a high-production protease Bacillus subtilis strain capable of degrading soybean antigenic protein in soybean meal

Method used

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  • Soybean antigenic protein degradation strain and application thereof
  • Soybean antigenic protein degradation strain and application thereof
  • Soybean antigenic protein degradation strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1 Bacillus subtilis ( Bacillus subtilis ) Obtaining of HN013 strain

[0014] 1. Medium

[0015] Enrichment medium: 3 g beef extract, 10 g peptone, 5 g NaCl, 1000 mL distilled water, adjust pH to 7.2; primary screening medium: 3 g beef extract, 10 g casein, 5 g NaCl, 20 g agar, 1000 mL distilled water , adjust the pH to 7.2; seed medium: glucose 10 g, peptone 5 g, yeast extract 5 g, KH 2 PO 4 1 g, MgSO 4 0.2 g, Na 2 CO 3 10 g, distilled water 1000mL, adjust pH to 7.2; fermentation medium: 40 g soybean meal, distilled water 1000mL, adjust pH to 7.2; solid slant medium: glucose 10 g, peptone 5 g, yeast extract 5 g, KH 2 PO 4 1 g, MgSO 4 0.2 g, Na 2 CO 3 10 g, 20 g agar, 1000 mL distilled water, adjust the pH to 7.2;

[0016] Preparation of separation medium (using soybean antigenic protein as the only nitrogen source): 5 g of soybean meal sample crushed through a 60-mesh sieve, mixed with 0.03 mol / L Tris-HC1 buffer solution (containing 0.01 mol / ...

Embodiment 2

[0025] Embodiment 2 Bacillus subtilis ( Bacillus subtilis ) Application of HN013 in fermented soybean meal

[0026] 1. Test material:

[0027] (1) Ordinary soybean meal;

[0028] (2) Bacillus subtilis ( Bacillus subtilis ) HN013 seed culture solution; Bacillus subtilis A1 (preserved in this laboratory, a strain producing fermented soybean meal, used as a control bacteria) seed culture solution.

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Abstract

The invention discloses a soybean antigenic protein degradation strain named as bacillus subtilis HN013. The strain is preserved in CGMCC (China General Microbiological Culture Collection Center) on the 24th of February in 2012, and the preservation number is CGMCC No.5788. The strain has the characteristic of being capable of degrading soybean antigenic protein and high-yield protease in bean pulp, and can be used for reducing the content of the soybean antigenic protein, increasing the content of soybean peptide in the fermented bean pulp and improving the application value of the bean pulp when being applied to the production of fermented bean pulp.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and relates to a soybean antigen protein-degrading bacillus strain and its application in the production of fermented soybean meal. technical background [0002] Soybean meal is a high-quality vegetable protein feed, which has the characteristics of high protein content, reasonable amino acid composition, and high utilization rate of animals. In recent years, it has been found that soybeans are rich in soybean isoflavones and other substances that can improve animal immunity, which is popular among people. However, the soybean antigen protein present in soybean meal is a heat-stable anti-nutritional factor, which seriously affects the health and growth rate of young animals such as piglets. , Disorders of absorption, leading to indigestion, diarrhea and stunted growth. It has been determined that the allergic reaction of piglets to soybean antigen protein is one of the main causes of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L1/20C12R1/125A23L11/00
Inventor 苏玉虹田玉民郭福来许云贺张莉力王安民韩丽娟
Owner 辽宁德宝农牧集团有限公司