VHH (variable domain of heavy chain of heavy-chain antibody) antibody gene derived from anti-CyPA (CyclophilinA) animal of family Camelidae, encoded polypeptide, and application thereof

A gene and animal technology, applied in the direction of anti-animal/human immunoglobulin, plant gene improvement, application, etc., can solve problems such as antibody drugs that have not been reported

Active Publication Date: 2012-09-12
FOURTH MILITARY MEDICAL UNIVERSITY
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports of antibody drugs targeting CypA

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • VHH (variable domain of heavy chain of heavy-chain antibody) antibody gene derived from anti-CyPA (CyclophilinA) animal of family Camelidae, encoded polypeptide, and application thereof
  • VHH (variable domain of heavy chain of heavy-chain antibody) antibody gene derived from anti-CyPA (CyclophilinA) animal of family Camelidae, encoded polypeptide, and application thereof
  • VHH (variable domain of heavy chain of heavy-chain antibody) antibody gene derived from anti-CyPA (CyclophilinA) animal of family Camelidae, encoded polypeptide, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] (1) Screening of anti-CypA heavy chain VHH antibody A1

[0018] 1. Immunity of alpacas

[0019] Two alpacas were immunized with CypA, and the immunization scheme was as follows: Alpaca antigen dosage for immunization: 0.5mg, 0.5mg, 1.0mg, 1.0mg, 2.0mg, 4.0mg, 4.0mg. The interval between immunizations was 2 weeks. Method: The first immunization was to grind and mix the antigen plus Freud's complete adjuvant, and inject it subcutaneously at multiple points. Subsequent immunization, antigen plus Freud's incomplete adjuvant, multi-point subcutaneous injection, 0.5ml / point, 4 points / time. Take a small amount of blood before each immunization, and use the ELISA method to detect the titer of anti-CypA antibody in alpaca blood, such as figure 1 As shown, after 7 times of immunization, the antibody titer in the blood of one alpaca reached 1:186,000.

[0020] 2. Construction of anti-cyclophilin A immune phage antibody library

[0021] The peripheral blood of immunized alpaca...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to VHH (variable domain of heavy chain of heavy-chain antibody) antibody gene derived from anti-CyPA (CyclophilinA) animal of family Camelidae, encoded polypeptide, and application of the gene and the polypeptide in preparing drugs for treating inflammatory diseases such as rheumatoid arthritis. The VHH antibody gene has gene sequence shown in sequence table (400)1, the encoded polypeptide has amino acid sequence shown in sequence table (400)2. Lama pacos as immune animal is used, and phage display techniques (PDT) are adopted to successfully obtain an anti-CyPA VHH antibody capable of specifically combining with expressed CyPA antigen. The antibody can inhibit monocytes THP-1 chemotaxis and gelatinase secretion induced by CyPA. Dimer or polymer can be constructed by gene engineering method, to be applied in diagnosis or research on drugs for treating inflammatory diseases. The gene and the polypeptide establish foundation for further research on related drugs for treating rheumatoid arthritis.

Description

Technical field [0001] The invention relates to the fields of biotechnology and medicine, especially the gene and polypeptide of alpaca heavy chain VHH antibody against cyclophilin A (cyclophilin A, CypA) antigen, and the application of the gene and polypeptide. Background technique [0002] The global incidence of rheumatoid arthritis (RA) is 1%, and there are more than 5 million RA patients in my country. The disability rate of RA patients is high. Within the first 2 years of onset, about 75% of patients have joint damage, and 80% of patients suffer from joint deformation and disability after 20 years. Some diseases progress rapidly and even lead to death. Therefore, the treatment of RA has always been the focus of medical attention. In recent years, RA treatment drugs have been continuously developed, especially the application of a series of new biological agents has significantly improved synovial inflammation in RA patients and slowed down joint damage. However, the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/13C07K16/18A61K48/00A61K39/395A61P29/00A61P19/02
Inventor 陈志南汪莉朱平马小魁李郁贾俊峰杨向民
Owner FOURTH MILITARY MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap