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Method for detecting raw pork and detection kit therefor

A technology of raw pork and detection department, which is applied in the field of immunochromatographic assay reagents, achieves the effect of stability and easy acquisition, no decrease in sensitivity, and low price

Inactive Publication Date: 2012-09-19
TANAKA PRECIOUS METAL IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the PCR method has high specificity, it takes about 3 hours for detection, and there is a problem that equipment such as a gene amplification device or a detection device is required

Method used

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  • Method for detecting raw pork and detection kit therefor
  • Method for detecting raw pork and detection kit therefor
  • Method for detecting raw pork and detection kit therefor

Examples

Experimental program
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Effect test

preparation example Construction

[0071] The production method of the polyclonal antibody of the present invention is as follows.

[0072] [Preparation of polyclonal antibody]

[0073] Rabbits were immunized with IgG (commercially available) contained in porcine serum according to a conventional method to obtain antiserum. Rabbit-derived anti-pig IgG antibody was purified from antiserum by salting out with 50% saturated ammonium sulfate, followed by purification and recovery with DEAE column (anion exchange column).

[0074] However, if this rabbit-derived anti-pig IgG antibody is directly used as a detection antibody in an immunochromatographic assay or an ELISA assay, although it will react significantly with raw pork, it will also react with a standard buffer (background), so it cannot Used as a detection antibody. Therefore, no implementation of beef, mutton, chicken. The test results are shown in "Table 1".

[0075] "HamaPhoto" in the table is an abbreviation for "Hamamatsu Densitometer".

[0076] [T...

Embodiment

[0101] The immunochromatography device used in the present invention will be described in detail below, but this is just an example, and the present invention is not limited thereto.

[0102] 1. Fabrication of reaction sites on chromatography media

[0103] On a nitrocellulose membrane (HF180 manufactured by Millipore) of 25 x 2.5 cm using an antibody coater (manufactured by BioDot Co., Ltd.), goat The anti-pig IgG antibody was dried at 42°C for 60 minutes and then at room temperature overnight to make reaction sites on a chromatography medium in which the carbonated buffer contained 5% by weight sucrose and 5% by weight isopropanol.

[0104] 2. Preparation of marker solution

[0105] 0.1 mL of goat-derived anti-pig IgG antibody diluted to a concentration of 0.05 mg / mL with HEPES buffer (pH 7.5) was added to 0.5 mL of colloidal gold suspension (manufactured by Tanaka Precious Metal Industry Co., Ltd., average particle size: 60 nm). The mixture was allowed to stand at room ...

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Abstract

Disclosed is a preparation of an optimal detection antibody for specifically detecting with high efficiency and high sensitivity, and without causing nonspecific reactions, raw pork in uncooked food by the immunoassay method; and a simple and highly accurate detection method using said detection antibody, and a test kit therefor. The antibody, which specifically recognizes immunoglobin G found in raw pork in uncooked food, is used as the detection antibody in an immunochromatographic detection method for proteins derived from raw pork in uncooked food, and an immunochromatographic detection device and kit for implementing same.

Description

technical field [0001] The invention relates to a method for detecting (raw) pork in non-heating food and a detection kit thereof. The present invention more particularly relates to an immunochromatographic assay for the detection of (raw) pork in non-heated foods using antibodies obtained by immunizing animals with immunoglobulin G in porcine serum that specifically recognize this immunoglobulin G reagent. Background technique [0002] The EU Scientific Operations Committee reported the risk of bovine infectious diseases such as bovine encephalic spongiform disease (BSE) to humans in its opinion. Since then, the use of meat and bone meal from BSE-infected cattle in livestock feed has received attention. In addition, the inspection of counterfeit food, such as the inspection of counterfeit food such as counterfeit brand beef that is regarded as a local brand, has also received a lot of attention. [0003] On the other hand, patients with food allergies may develop various ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/12G01N33/543
CPCG01N2333/70503C07K16/4241G01N33/12G01N33/6854
Inventor 榊原雄広望月一芳芝井勇亮岩本久彦
Owner TANAKA PRECIOUS METAL IND
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