Four types of sulfuric acid nepresol organic drug eutectics and preparation method thereof
A kind of technology of bishydralazine and organic medicine, applied in the field of bishydralazine sulfate organic medicine co-crystal and preparation thereof
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Embodiment 1
[0054] Synthesis of organic drug cocrystals using dihydralazine sulfate and 2,3-dihydroxybenzoic acid 1:
[0055] Raw material handling:
[0056] Accurately weigh 0.2mmol of hydralazine sulfate with an analytical balance, add 3ml of 2mol / L NaOH solution, stir for 30 minutes, then put the cloudy solution into a 5ml centrifuge tube, and centrifuge at a speed of 12000r / min for 3 minutes. The supernatant was removed, washed with water three times repeatedly, and put into a vacuum drying oven for drying.
[0057] Weighing:
[0058] The reactants are fed according to the ratio of dihydralazine: 2,3-dihydroxybenzoic acid = 1:2, and 0.2mmol dihydralazine and 0.4mmol 2,3-dihydroxybenzoic acid are accurately weighed with an analytical balance. Formic acid, all placed in clear glass vials.
[0059] Dissolution of API:
[0060] Accurately pipette 2ml of ethanol and 2ml of tetrahydrofuran into the glass vial with a 5ml pipette gun, stir for 30 minutes, the solution is in the state of w...
Embodiment 2
[0064] Synthesis of Organic Drug Cocrystal 2 Using Dihydralazine Sulfate and 2,4-Dihydroxybenzoic Acid:
[0065] Raw material handling:
[0066] Accurately weigh 0.2mmol of hydralazine sulfate with an analytical balance, add 3ml of 2mol / L NaOH solution, stir for 30 minutes, then put the cloudy solution into a 5ml centrifuge tube, and centrifuge at a speed of 12000r / min for 3 minutes. The supernatant was removed, washed with water three times repeatedly, and put into a vacuum drying oven for drying.
[0067] Weighing:
[0068] The reactants are fed according to the ratio of dihydralazine: 2,4-dihydroxybenzoic acid = 1:2, and 0.2mmol dihydralazine and 0.4mmol 2,4-dihydroxybenzoic acid are accurately weighed with an analytical balance. Formic acid, all in clear glass vials.
[0069] Dissolution of API:
[0070] Accurately pipette 1ml of ethanol and 3ml of tetrahydrofuran into the glass vial with a 5ml pipette gun, and stir for 30 minutes. The solution is in the state of white...
Embodiment 3
[0074] Synthesis of Organic Drug Cocrystal 3 Using Dihydralazine Sulfate and 2,5-Dihydroxybenzoic Acid:
[0075] Raw material handling:
[0076] Accurately weigh 0.2mmol of hydralazine sulfate with an analytical balance, add 3ml of 2mol / L NaOH solution, stir for 30 minutes, then put the cloudy solution into a 5ml centrifuge tube, and centrifuge at a speed of 12000r / min for 3 minutes. The supernatant was removed, washed with water three times repeatedly, and put into a vacuum drying oven for drying.
[0077] Weighing:
[0078] The reactants are fed according to the ratio of dihydralazine: 2,5-dihydroxybenzoic acid = 1:2, and 0.2mmol dihydralazine and 0.4mmol 2,5-dihydroxybenzoic acid are accurately weighed with an analytical balance. Formic acid, all in clear glass vials.
[0079] Dissolution of API:
[0080] Accurately pipette 2ml of ethanol and 2ml of tetrahydrofuran into the glass vial with a 5ml pipette gun, stir for 30 minutes, the solution is in the state of white tur...
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