Coumarin from Cichorium plants used for preparing drug with liver-protecting effect, and extraction and purification method of coumarin from Cichorium plants

Abstract

The invention provides a coumarin from Cichorium plants used for preparing drug with liver-protecting effect, and an extraction and purification method of the coumarin from Cichorium plants. The coumarin from Cichorium plants is characterized in that determined by an ultraviolet spectrophotometric method at wavelength of 350 nm with esculetin (6,7-dihydroxycoumarin) as reference substance, the coumarin has purity up to 90%. The method takes Cichorium glafulosum boiss. et Huet or Cichorium intybus L. as materials; combines ethanol extraction technology, macroporous adsorption resin enrichment technology, vacuum evaporation technology, ultrasonic extraction technology, preparative high performance liquid chromatographic (HPLC) purification technology and vacuum lyophilizing technology, to obtain the coumarin with high purity, safety, effectiveness, controllable quality and liver-protecting effect. The coumarin can be used for preparing liver-protecting drug or health food.

Description

technical field [0001] The invention relates to the technical field of purified plant extracts and their application to medicines or health care products, and relates to chicory coumarin, which is a medicine with liver-protecting effect, and its extraction and purification method. Background technique [0002] In China, there are two species of Chicory (Cichorium glafulosum boiss.et Huet) and Chicory (Cichorium intybus L.), which are commonly used medicinal materials in Uyghur (Uyghur name: Kasen). Chicory likes temperature and cold resistance, and has no strict requirements on soil. It is distributed in Northwest China, North China and Northeast China, mainly in the southern part of Xinjiang, China (Liu Yongmin. Uyghur Medicine Chronicles (Part 1) [M]. Urumqi. Xinjiang People's Publishing House , 1999:47). Uighur medicine in Xinjiang, China mainly uses the aerial part of chicory as medicine. It tastes slightly bitter, salty, and cool in nature. It has the functions of clea...

AI Technical Summary

Problems solved by technology

[0005] In recent years, although various Uyghur medicine manufacturers in Xinjiang and Uyghur medical hospitals in various regions have produced dozens of preparations using chicory as the main medicinal material, due to the generally backward production technology of these preparations (only water extraction is carried out, the impurities contained are many , taking a large amount), there is no specific content determination method and quality control index for controlling the quality of chicory, and the functions and indications are too broad to make it difficult to clearly locate clinically, so it cannot meet the requirements of the national new drug standard, let alone enter the international market. , has greatly affected the utilization and promotion of chicory

At present, the research on the effective part of chicory mainly adopts organic solvents such as ethanol, ethyl acetate, n-butanol, n-hexane, petroleum ether, and chloroform to extract and purify. These traditional purification methods have the disadvantages of energy consumption, time consumption, high cost, low purity, The pollution is large and causes shortcomings such as organic solvent residues (Du Haiyan, Yuan Sitong, Jiang Peifen. Research on the chemical constituents of chicory. Chinese Journal of Traditional Chinese Medicine, 1998, 23 (11): 682; Zheng Hongmei, Wang Daguang. Chicory n-hexane extract on blood sugar, Effects of blood lipids [J]. Chinese Journal of Traditional Chinese Medicine Information, 2000, 4 (7): 38-39; Xu Yamei, Hu Tianming. Study on antibacterial activity of chicory root extracts [J]. 3): 0615-0619)