Protein microdialyzer and usage method

Abstract

The invention provides a protein microdialyzer and a usage method. The microdialyzer comprises a floating plate 1, a micro-dialyzing pipe component 14, a semipermeable membrane 11 and a magnetic stirrer 8. The micro-dialyzing pipe component is composed of an outer sleeve 9 and an inner sleeve 10; the outer sleeve 9 is shaped like a sleeve in which upper part is bigger than lower part; the inner sleeve 10 is shaped like a straight barrel; an external thread is formed at the top of the inner sleeve 10, and adapts to the inner thread of a pipe cover 13. The floating plate 1 is a round porous plastic plate; a circle of apron 5 is formed at the bottom of each round hole; and a strechable shaft rod 6 is arranged in the middle lower part of the floating plate 1. The usage method of the microdialyzer is as follows: using the semipermeable membrane 11 to cover the bottom of the inner sleeve 10 and using a rubber band 12 to bind the bottom of the inner sleeve 10; inserting the inner sleeve into the outer sleeve 9; then adding a protein sample to the semipermeable membrane 11 at the bottom of the inner sleeve; covering the pipe cover 13; inserting the whole component 14 into the corresponding inserting holes 2, 3 or 4 of the floating plate; pulling out the shaft rod 6 from the bottom of the floating plate; regulating the length of the shaft rod according to height of the buffer solution, and adsorbing the magnetic stirrer 8 under an adsorption cover 7; and finally, floating the whole device on the surface of the buffer solution for micro-dialyzing.

Description

technical field [0001] The invention belongs to the experimental technical field of protein purification, and in particular relates to a new protein microdialysis device and its application. Background technique [0002] Protein purification has always been a very important technical content in biochemical research, among which protein separation and desalting involve a series of important experimental techniques. Usually, in the laboratory, proteins are purified by desalting methods such as dialysis, Sephadex G-25 column chromatography, reversed-phase column chromatography, ultrafiltration, etc. However, these experimental methods have their own advantages and disadvantages. [0003] Specifically, desalting with Sephadex G-25 chromatographic column is fast and the amount of sample to be processed is large, but it will bring about a large increase in the volume of the eluent. Therefore, concentration and freeze-drying after desalting are often required. It may be feasible f...

AI Technical Summary

Problems solved by technology

However, the problem is that this technology also has its shortcomings in the application process

In the laboratory, researchers often experience that adding and removing protein samples to and from dialysis bags is a time-consuming and troublesome operation

Because the dialysis bag does not have any rigidity to support, for unskilled operators, in the process of adding or taking out the sample, or tightening the mouth of the dialysis bag with a thread, the sample may be lost accidentally

In the experiment, leakage often occurs because one end of the dialysis bag is not tightly fastened. Therefore, if the protein sample to be dialyzed is very precious, the volume is small, and the natural activity of the protein needs to be maintained (for example, less than 1 ml). , using traditional dialysis methods for desalting, the protein will be difficult to recover

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