Radix polygonati officinalis microsatellite DNA (deoxyribonucleic acid) molecular marker

A DNA molecule, microsatellite marker technology, applied in recombinant DNA technology, DNA/RNA fragment, determination/inspection of microorganisms, etc., can solve the problems of few researches on molecular breeding and low reproduction coefficient of Polygonatum japonica

Inactive Publication Date: 2012-10-03
ANHUI NORMAL UNIV
View PDF8 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, a series of problems have appeared in the cultivation and domestication process of Polygonatum odoratum, such as low reproductive coefficient, and there are few studies on molecular breeding of Polygonatum odoratum using microsatellite markers.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Radix polygonati officinalis microsatellite DNA (deoxyribonucleic acid) molecular marker
  • Radix polygonati officinalis microsatellite DNA (deoxyribonucleic acid) molecular marker
  • Radix polygonati officinalis microsatellite DNA (deoxyribonucleic acid) molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Construction of Polygonatum Polygonatum Microsatellite DNA Enrichment Library

[0025] 1) Genome extraction and digestion:

[0026] Using the CTAB method introduced by Doyle J (Doyle J. DNA protocols for plants-CTAB total DNA isolation [A]. In Hewitt GM, Johnston A (eds.), Molecular Techniques in Taxonomy [M]. Berlin: Springer, 1991, 283 -293) to extract the genome. The genome was digested with restriction endonuclease Sau 3AI. After 2% agarose gel electrophoresis, the DNA fragments of 300-900 bp were excised under ultraviolet light. Recover with gel recovery kit.

[0027] 2) Add connectors:

[0028] Add the gel recovered and purified product to the adapter:

[0029] oligo A (5'-GGCCAGAGACCCCCAAGCTTCG-3')

[0030] oligo B (5’-PO 4 -GATCCGAAGCTTGGGGTCTCTGGCC-3'),

[0031] Under the action of T4 DNA Ligase, they were ligated overnight in a water bath at 16°C.

[0032] 3) PCR detection adapter connection:

[0033] Use oligo A as primer and ligation product as tem...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a radix polygonati officinalis microsatellite DNA molecular marker, comprising the steps of constructing a radix polygonati officinalis microsatellite (CT) n enrichment library; screening and sequencing microsatellite sequence positive clone; obtaining 55 clones containing the microsatellite repeated sequence; and screening to obtain 9 high polymorphic microsatellite molecular markers Po1, Po2, Po3, Po4, Po5, Po6, Po7, Po8 and Po9. The radix polygonati officinalis microsatellite DNA molecular marker can be used for researching genetic diversity of the radix polygonati officinalis, variation and evolutionary relationship between polygonatum plants and population, has good repeatability and is a reliable and efficient molecular marker.

Description

technical field [0001] The invention relates to a molecular marker technology, in particular to a microsatellite molecular genetic marker of polygonatum polygonatum. Background technique [0002] Microsatellites are also called short tandem repeats (Short Tandem Repeats, STRs), simple sequence repeats (Simple Sequence Repeat, SSRs), simple sequence length polymorphism (SSLP). It refers to a kind of simple tandem repeat consisting of several nucleotide pairs (called core sequence, generally 1-6 bps) as the repeating unit throughout the biological genome. The number of repeats is highly variable among different genotypes of the same species, but the two ends of this repeat are relatively conserved single-copy sequences. Based on this, primers can be designed to analyze the variation of the repeat number of the core sequence by PCR , polymorphisms were detected among different genotypes. According to the composition of repeating units, microsatellite DNA sequences are divided...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 朱国萍高鹏王晖郑基阳李雪王文才唐王刚赵晓宇
Owner ANHUI NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap