Feeding method for improving rifamycin SV fermentation yield

Abstract

The invention discloses a feeding method for improving rifamycin SV fermentation yield. In a fermentation culture process, alanine with final concentration of 0.3-0.8 g / L, bean oil with final concentration of 2-5 mL / L and fosfomycin sodium with final concentration of 3-20 mg / L or alanine with final concentration of 0.3-0.8 g / L, bean oil with final concentration of 2-5 mL / L and fosfomycin trometamol with final concentration of 3-20 mg / L are fed into a three-stage fermentation tank with amycolatopsis mediterranei fermentation culture period of 60-80 hours in a stile way, wherein the rifamycin SV titer at the end of the culture is averagely increased by 17.0 percent. Feeding substances used by the method are all industrial and agricultural bulk products. The feeding method has the advantages of high affordability, simple operation process, high controllability, suitability for large-scale fermentation production, stable production increase effect and capability of meeting the environment-friendly development requirements on energy conservation and consumption reduction of the fermentation industry of the country.

Description

technical field [0001] The invention relates to a preparation method of rifamycin, in particular to a feeding method for improving the fermentation yield of rifamycin SV. Background technique [0002] As a bulk antibiotic raw material for tuberculosis treatment, the fermentation unit of domestic rifamycin SV has been hovering around 5000 U / mL. The physiological state of the bacteria at the industrial production level is closely related to the process control. There is an interaction effect among the glycolysis, pentose phosphate pathway, tricarboxylic acid cycle pathway and rifamycin precursor synthesis pathway. It is determined that the latter is related to the specific The corresponding relationship of metabolic bottleneck will guide the optimization index and specificity of rifamycin SV fermentation production process, which has important practical significance for improving the control and execution of production efficiency. [0003] The carbon and nitrogen metabolic pa...

AI Technical Summary

Problems solved by technology

However, according to scientific and technological research, it is related to the optimal concentration of alanine, soybean oil and fosfomycin sodium or alanine, soybean oil and fosfomycin trometamol at the optimal time to supplement Amycolatopsis mediterranei In the fermentation culture, the research on regulating the synthetic metabolic flow of rifamycin SV precursor and improving its synthesis efficiency has not yet seen relevant literature and published patent reports