Feeding method for improving rifamycin SV fermentation yield

A technology of rifamycin and yield, applied in the field of feeding material for improving rifamycin SV fermentation yield, can solve problems such as no relevant literature and published patent reports, and achieves strong commoditization, stable production increase effect, and easy operation. simple craftsmanship

Inactive Publication Date: 2012-10-03
河南省南街村(集团)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, according to scientific and technological research, it is related to the optimal concentration of alanine, soybean oil and fosfomycin sodium or alanine, soybean oil and fosfomycin trometamol at the optimal time to supplement Amycolatopsis mediterranei In the fermentation culture, the research on regulating the synthetic metabolic flow of rifamycin SV precursor and improving its synthesis efficiency has not yet seen relevant literature and published patent reports

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1 Fermentation culture

[0018] Prepare the culture medium according to the formula of seeds and fermentation medium, cool down to 28°C after damp heat sterilization, and transfer the seed liquid of Amycolatopsis mediterranei into the first-level seed tank according to the inoculum amount of 3%-5%. The temperature of the primary seed tank is controlled at 28°C, the sterile air flow rate is controlled at 0.8 vvm, the stirring speed is 160 r / min, the tank pressure is controlled at 0.03-0.04 MPa, and the secondary seed tank is filled into the secondary seed tank by aseptic operation after 48 hours of cultivation; The temperature of the first-grade seed tank is controlled at 28°C, the sterile air flow rate is controlled at 0.8 vvm, the stirring speed is 160 r / min, and the tank pressure is controlled at 0.03-0.04 MPa. After 48 hours of cultivation, the aseptic operation is added to the third-grade fermenter; the third-grade The temperature of the fermenter is controlled at 2...

Embodiment 2

[0022] 1 Fermentation culture

[0023] Same as Example 1

[0024] 2 feeding process

[0025] When the fermentation period was 80 h, alanine with a final concentration of 0.8 g / L, soybean oil at 2 mL / L and fosfomycin sodium at 20 mg / L were aseptically fed into the three-stage fermenter.

Embodiment 3

[0027] 1 Fermentation culture

[0028] Same as Example 1

[0029] 2 feeding process

[0030] When the fermentation period was 70 h, alanine at a final concentration of 0.5 g / L, soybean oil at 3.5 mL / L and fosfomycin sodium at 10 mg / L were fed into the three-stage fermentation tank.

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Abstract

The invention discloses a feeding method for improving rifamycin SV fermentation yield. In a fermentation culture process, alanine with final concentration of 0.3-0.8 g/L, bean oil with final concentration of 2-5 mL/L and fosfomycin sodium with final concentration of 3-20 mg/L or alanine with final concentration of 0.3-0.8 g/L, bean oil with final concentration of 2-5 mL/L and fosfomycin trometamol with final concentration of 3-20 mg/L are fed into a three-stage fermentation tank with amycolatopsis mediterranei fermentation culture period of 60-80 hours in a stile way, wherein the rifamycin SV titer at the end of the culture is averagely increased by 17.0 percent. Feeding substances used by the method are all industrial and agricultural bulk products. The feeding method has the advantages of high affordability, simple operation process, high controllability, suitability for large-scale fermentation production, stable production increase effect and capability of meeting the environment-friendly development requirements on energy conservation and consumption reduction of the fermentation industry of the country.

Description

technical field [0001] The invention relates to a preparation method of rifamycin, in particular to a feeding method for improving the fermentation yield of rifamycin SV. Background technique [0002] As a bulk antibiotic raw material for tuberculosis treatment, the fermentation unit of domestic rifamycin SV has been hovering around 5000 U / mL. The physiological state of the bacteria at the industrial production level is closely related to the process control. There is an interaction effect among the glycolysis, pentose phosphate pathway, tricarboxylic acid cycle pathway and rifamycin precursor synthesis pathway. It is determined that the latter is related to the specific The corresponding relationship of metabolic bottleneck will guide the optimization index and specificity of rifamycin SV fermentation production process, which has important practical significance for improving the control and execution of production efficiency. [0003] The carbon and nitrogen metabolic pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/18C12R1/01
Inventor 李盘欣王志黄亚男马向东史咏华李晓铃彭丽王培英林乐
Owner 河南省南街村(集团)有限公司
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