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Separate culture and preservation method of russula vinosa mycorrhizal type edible fungus

A technology of separation culture and preservation method, which is applied in the field of separation culture and preservation of russula mycorrhizal edible fungus, which can solve the problems of low purification success rate, difficult and long-term preservation of strains, and low germination rate of russula russula isolation culture , to achieve the effect of simple operation process and simple storage method

Inactive Publication Date: 2013-06-19
RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Researchers engaged in the research of edible fungi generally feel that the germination rate of the isolation and culture of russula is low, the success rate of purification is low, the storage time of the strain is difficult to last, and the activity of the strain disappears quickly

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for isolating, cultivating and preserving russula mycorrhizal edible fungi, the steps are as follows:

[0024] (1) Formulated with folic acid and vitamin B 1 and lactose-modified PDA medium.

[0025] The composition of the improved PDA medium is: potato juice 200g / L, glucose 20g / L, lactose 5g / L, folic acid 50μg / L, vitamin B 1 100μg / L, agar powder 8g / L.

[0026] (2) Put the culture medium prepared in step (1) into the Erlenmeyer flask and sterilize it at 121°C for 20 minutes, take it out until the temperature drops to about 50°C, put it into a sterile culture medium at 30ml / dish under a clean workbench Put it in a dish (2×9cm), put it in the workbench and cool it into a flat solid medium, and set it aside.

[0027] (3) Under the clean workbench, use sterile tweezers to take a small piece of gills at the junction of the stipe and the cap of the fruiting body of the russula officinalis, and quickly put it into the petri dish prepared in step (2) , The gills ar...

Embodiment 2

[0039] Except that the concentration of lactose in the medium of step (1) is 4g / L, the concentration of lactose in the medium of step (5) is 0.5g / L, and the two kinds of medium are mixed in the ratio of 1:1 in step (9) Except, other operations are all identical with embodiment 1.

[0040] Adopt the condition of embodiment 2, the success rate of strain separation of Russula russula reaches 40%, and the purification rate reaches 60%, and the substratum in the test tube keeps the original state substantially after strain storage 12 months, and strain activity reaches 70%; Preserves 24 months After one month, the culture medium in the test tube was reduced by 9%, and the strain activity reached 50%.

Embodiment 3

[0042] Except that the concentration of lactose in the medium of step (1) is 6g / L, the concentration of lactose in the medium of step (5) is 1.5g / L, and the two kinds of medium are mixed in the ratio of 1:1 in step (9) Except, other operations are all identical with embodiment 1.

[0043] Adopting the conditions of embodiment 3, the success rate of strain separation of Russula officinalis reaches 50%, and the purification rate reaches 50%. After 12 months, the culture medium in the test tube decreases by 10%, and the strain activity reaches 80%; After one month, the culture medium in the test tube was reduced by 20%, and the strain activity reached 70%.

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Abstract

The invention discloses a separate culture and preservation method of russula vinosa mycorrhizal type edible fungus, which comprises the following steps: picking fresh and mature russula vinosa sporocarp; taking tissue blocks at a gill part of the junction of a stipe and a pileus on the russula vinosa sporocarp, placing the tissue blocks in an improved PDA solid medium containing lactose, vitaminB1 and folic acid for light-tight culture for 3-5 days at room temperature, and germinating to obtain mycelium; placing the mycelium in an improved MN solid medium containing lactose for light-tight culture for 5-7 days at room temperature, and purifying the mycelium; transferring the purified mycelium into a test tube with a rubber cover for light-tight culture for 5-7 days at room temperature; and placing the pollution-free test tube in an environment at 10-15 DEG C for preservation. In the separate culture and preservation method of russula vinosa mycorrhizal type edible fungus, the germination rate of the mycelium of the tissue blocks is as high as above 50%, one-time transferring of the strain preservation time can reach 1 year, and the strain activity lasts for 3 years without degeneration.

Description

technical field [0001] The invention relates to the technical field of symbiotic microorganism cultivation, in particular to a method for separating, cultivating and preserving russula mycorrhizal edible fungi. Background technique [0002] Mycorrhizal edible fungi refer to a class of edible fungi that must rely on some living tree roots in their own life history and form a symbiotic relationship with tree roots. The nutritional physiological process between mycorrhizal edible fungi and tree roots first forms a symbiont-mycorrhiza, in order to complete the interdependence of material exchange. Among the 567 years of wild edible fungi resources known in my country, there are 352 species of mycorrhizal edible fungi, accounting for about 53.6% of the total domestic edible fungus resources. Many kinds of mycorrhizal edible fungi have high economic and nutritional value, and they are also of great significance to the sustainable development of forests. [0003] Russula is a new...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14
Inventor 陈羽仲崇禄姜清彬张勇陈珍
Owner RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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