Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for in vitro preservation of betel nut genetic resources

An in vitro preservation and germplasm technology, applied in the field of plant cell engineering, can solve the problems of high cost and large area for field preservation, and achieve the effects of simple process, avoiding the spread of diseases and insect pests, and delaying the growth rate.

Inactive Publication Date: 2012-10-17
COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for the in vitro preservation of betel nut germplasm resources. By increasing the amount of sugar in the preservation medium, the growth rate of the plants can be well delayed, which is beneficial to the exchange of germplasm resources at home and abroad, and avoids the in vitro preservation of betel nut germplasm resources. The spread of diseases and insect pests occurs in the process of mass exchange, and at the same time, it can solve the problems of large area and high cost of field preservation, provide theoretical support for in vitro rapid propagation technology, and provide an effective way for the collection and preservation of betel nut germplasm resources and the industrial production of seedlings. way

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for in vitro preservation of betel nut genetic resources

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1. Embryo acquisition and pre-culture

[0015] Select the germplasm resource result trees over 15 years old for follow-up investigation, and after determining the target plant, harvest in time from the time when the female flower is pollinated to 7-8 months after the fruit (the maturity of the betel nut embryo affects the germination rate of the embryo, and the maturity of the embryo The higher the seedling rate is, the higher the seedling rate is. Generally, betel nut fruit matures for 12 months, but the 12-month betel nut seed fruit is not conducive to the acquisition of seed embryos. Because the endosperm is as hard as a stone when it matures, it is difficult to get the embryo. Embryos are easy to obtain and culture at 8 months). After collection, the fruit should be rinsed with running water. After the pericarp is cut off, the endosperm block covered with the embryo is left, the size is about 2×2cm. The endosperm block containing the embryo is washed with tap water,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the plant cells engineering technical field, and relates to a method for in vitro preservation of betel nut genetic resources. The method is characterized in that germplasm resource betel nut trees with 15 years growth are selected for planting, embryo is removed to place in a pre-culture medium for culturing, the embryo is germinated to complete plants, when the plant is grown to the height with 1.5-2 cm and placed in a preservation medium for preserving and culturing to realize the in vitro preservation of the betel nut genetic resources. The method has the advantages of simple technology and strong operationality, by raising the sugar amount preserved in the medium, the growth speed of the betel nut plant can be prolonged, The method is in favor of preservation of germplasm resources, and is in favor of exchange of germplasm resources at home and abroad, the generation of spread of insect diseases in the germplasm exchange process can be avoided, and the problems of large land occupation, high cost and the like can be solved, and a theoretical support is provided for in vitro rapid propagation technology. The method of the invention can provide an effective approach for collection and preservation of the betel nut germplasm resources and industrial production of sprout.

Description

technical field [0001] The invention belongs to the technical field of plant cell engineering, and relates to a method for in vitro preservation of resources of palmaceae plants, in particular to a method for in vitro preservation of areca nut germplasm resources. Background technique [0002] Betel nut (Areca catechu L.) belongs to the genus Areca of the palm family. It is the main economic crop and tropical landscaping tree species in tropical areas of my country. It is also one of the important resources of southern medicine in my country. high economic value. In Asia and the Pacific region, areca nuts are mainly used as chewing products for local people, and betel nut cultivation has gradually developed towards commercialization and large-scale development. Areca nuts are mainly distributed in Hainan Province in my country, and the total output accounts for more than 99% of the areca nut production in the mainland. At present, the planting area in Hainan has reached 1.0...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
Inventor 黄丽云周焕起范海阔李杰刘立云
Owner COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com