Rapid two-step tissue culture propagation method of China fir based on LED (light emitting diode) light source

A LED light source, tissue culture rapid propagation technology, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve problems such as shortening rooting time, achieve shortening growth cycle, save power consumption, improve seedling growth speed and The effect of rooting speed

Inactive Publication Date: 2012-10-17
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The development of plant tissue culture and rapid propagation technology has gradually matured so far, but there are still many problems, especially the research on Chinese fir tissue culture and rapid propagation technology has been at a stagnant stage in recent years, and an effective method that can improve tissue culture has not been found. Seedling quality, shorten the growth period of seedlings, and reduce the cost; and Chinese fir tissue culture rooting needs to improve the quality of the root system and shorten the rooting time on the one hand, on the other hand, it is necessary to reduce the interference of callus in the rooting process of Chinese fir, so as to improve the quality of transplanting. Survival rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A. Seedling succession stage

[0020] (1) Preparation of tissue culture seedlings: select the shoot tip of tissue culture seedlings of excellent Chinese fir clones and inoculate them into the subculture medium;

[0021] (2) Light treatment: Subculture the subcultured seedlings under the red LED light source for subculture, and the light treatment is 12 h / d; the tissue cultured seedlings treated with the red light source directly enter the rooting stage, and need to continue the subcultured tissue culture The seedlings need to be rejuvenated after being treated with red light, and then placed in a blue LED light source;

[0022] B. Seedling rooting stage

[0023] (1) Preparation stage of tissue-cultured seedlings: select excellent Chinese fir clone seedlings subcultured under red LED light, and inoculate them in the rooting medium;

[0024] (2) Lighting treatment: put the rooted tissue culture seedlings in the blue LED light source for rooting culture, and the light ti...

Embodiment 2

[0029] A. Seedling succession stage

[0030] (1) Preparation of tissue culture seedlings: select the shoot tip of tissue culture seedlings of excellent Chinese fir clones and inoculate them into the subculture medium;

[0031] (2) Light treatment: Subculture the subcultured seedlings under the red LED light source for subculture, and the light treatment was 12 h / d; After the seedlings are treated with red light, they are placed in a blue LED light source for rejuvenation;

[0032] B. Seedling rooting stage

[0033] (1) Preparation stage of tissue-cultured seedlings: select excellent Chinese fir clone seedlings subcultured under red LED light, and inoculate them in the rooting medium;

[0034] (2) Lighting treatment: put the rooted tissue culture seedlings in the blue LED light source for rooting culture, and the light time is 12 h / d;

[0035] C. Seedling transplanting stage

[0036] When the average length of the roots of the rooted seedlings reaches about 2-4 cm, the seed...

Embodiment 3

[0039] A. Seedling succession stage

[0040] (1) Preparation of tissue culture seedlings: select the shoot tip of tissue culture seedlings of excellent Chinese fir clones and inoculate them into the subculture medium;

[0041] (2) Light treatment: Subculture the subcultured seedlings under the red LED light source for subculture, and the light treatment is 12 h / d; the tissue cultured seedlings treated with the red light source directly enter the rooting stage, and need to continue the subcultured tissue culture The seedlings need to be rejuvenated after being treated with red light, and then placed in a blue LED light source;

[0042] B. Seedling rooting stage

[0043] (1) Preparation stage of tissue-cultured seedlings: select excellent Chinese fir clone seedlings subcultured under red LED light, and inoculate them in the rooting medium;

[0044] (2) Lighting treatment: put the rooted tissue culture seedlings in the blue LED light source for rooting culture, and the light ti...

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Abstract

The invention discloses a rapid two-step tissue culture propagation method of China fir based on LED (light emitting diode) light source. The method adopts staged treatment with red / blue LED combined light source with adjustable light intensity: firstly monochromic red light (with main wavelength of 634 nm and spectrum range of 600-665 nm) is taken as China fir tissue culture light source to improve growth speed of tissue-cultured seedlings; then monochromic blue light (with main wavelength of 460 nm and spectrum range of 425-495 nm) is taken as light source to effectively promote rooting andseedling hardening. The method can improve China fir seedling growth speed and seedling robustness, shorten growth period, shorten rooting time, improve root number and vitality, improve transplantation survival rate, save electricity, improve China fir tissue-cultured seedling quality while greatly lower cost. The invention provides a novel method for rapid tissue culture propagation research ofChina fir.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture and rapid propagation, and in particular relates to a two-step method for tissue culture and rapid propagation of Chinese fir based on an LED light source. Background technique [0002] The development of plant tissue culture and rapid propagation technology has gradually matured so far, but there are still many problems, especially the research on Chinese fir tissue culture and rapid propagation technology has been at a stagnant stage in recent years, and an effective method that can improve tissue culture has not been found. Seedling quality, shorten the growth period of seedlings, and reduce the cost; and Chinese fir tissue culture rooting needs to improve the quality of the root system and shorten the rooting time on the one hand, on the other hand, it is necessary to reduce the interference of callus in the rooting process of Chinese fir, so as to improve the quality of transplant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 丁国昌康俊勇林思祖吴志明周锦业
Owner FUJIAN AGRI & FORESTRY UNIV
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