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Optical microscopic imaging system and imaging method

An imaging system, optical microscopy technology, applied in the field of optical microscopy

Inactive Publication Date: 2012-10-24
HEBEI UNIV OF ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, there is no detailed research report on the recording and reproduction of this digital holographic system.

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  • Optical microscopic imaging system and imaging method
  • Optical microscopic imaging system and imaging method
  • Optical microscopic imaging system and imaging method

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Embodiment Construction

[0023] figure 1 Schematic diagram of the optical microscopy imaging system. The 632.8nm laser light emitted by He-Ne laser 1 is divided into beam A and beam B after passing through half-wave plate 2 and polarization beam splitting prism 3, and after passing through beam expander collimator 4, one beam forms an illumination The plane light wave of sample 6 (transparent object) and passed through the microscope objective MO 1 (7) After forming an enlarged object light wave, another beam passes through the microscope objective lens MO 2 (8) After forming a divergent spherical reference light wave, the two beams of light are combined by the prism BS (ie, the beam combiner 9) and interfere, and are recorded by the CCD sensor 10 located in the image plane of the sample, and then sent to the computer 11 for processing , 5 in the figure represents the mirror. To eliminate MO 1 Introduced secondary phase distortion, MO 2 Should be with MO 1 It is exactly the same, and its position must ...

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Abstract

The invention relates to an optical microscopic imaging system and imaging method, belonging to field of optical microscopes. The system comprises a laser, a half-wave plate, a polarization beam splitter prism, a beam expander collimator, a microscope objective, a reflective mirror, a beam combining mirror, a CCD (Charge Coupled Device) and a computer. After the laser emits laser light, the polarization beam splitter prism divides the laser light into two light beams, and one of the light beams transmits a transparent object. The two light beams pass through the beam combining mirror and interfere with each other to form an image on the CCD, and the CCD transmits the obtained image into the computer. A microscopic strength image and a phase image of the object are obtained through digital reconstruction in the computer, so that a three-dimensional microscopic of the object is obtained.

Description

Technical field [0001] The application relates to a microscopic imaging system and a method for obtaining images through the system, and belongs to the field of optical microscopes. Background technique [0002] Traditional microscopic imaging is mainly obtained through lens groups, and the microscopic image obtained in this way is only the intensity image of the imaged object. With the continuous development of semiconductor technology and laser technology, a digital holography has emerged at this stage, which can obtain the phase image of the imaged object while obtaining the intensity image of the imaged object, or it can obtain the three-dimensional image of the imaged object . [0003] Digital holography uses a photoelectric conversion device (CCD or CMOS) to replace the traditional holographic dry plate to record interference fringes, and then send it to a computer to reconstruct the intensity and phase distribution of the object through numerical calculation. Compared with...

Claims

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Application Information

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IPC IPC(8): G03H1/12G02B21/36
Inventor 王华英刘飞飞于梦杰
Owner HEBEI UNIV OF ENG