Application of artemisinin and derivatives thereof in preparation of medicaments for treating hepatitis C viruses
A technology of hepatitis C virus and hepatitis C, which is applied in the field of medicine and can solve problems such as the application of artemisinin and its derivatives to hepatitis C virus.
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Embodiment 1
[0050] Embodiment 1: the evaluation of artemisinin and derivative thereof anti-hepatitis C virus activity
[0051] A kind of application of artemisinin and its derivatives in the preparation of medicine for treating hepatitis C virus (HCV), the steps are:
[0052] 1. Experimental materials
[0053] 1.1 Cells, plasmids, viruses and drugs
[0054]Huh7.5.1 cells were donated by Dr.F.V.Chisari (please refer to the table of genetic resources); the plasmid pJFH1 containing the complete genome sequence of HCV type 2a JFH1 strain was donated by Prof. Dr. Takaji Wakita (please refer to the table of genetic resources). The virus JFH1-5AGFP with reporter gene was prepared by our laboratory; artemisinin, dicyanide artemisinin, artemether and artesunate were purchased from sigma company.
[0055] 1.2 Reagents
[0056] DMEM medium was purchased from GIBCO Company; MTT detection reagent was purchased from biomol Company; restriction endonuclease was purchased from NEB Company.
[0057] 1...
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