Method for extracting alcohol-soluble antioxidant substances from visceral organs of abalone
A technology of antioxidant substances and extraction methods, applied in the direction of antivirus, food science, drug combination, etc., to achieve the effect of improving product quality, high antioxidant activity, and no loss of nutrients
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Embodiment 1
[0025] At 20°C, take 10 kg of abalone viscera that has been cleaned and removed of connective tissue, add 20 kg of distilled water, beat the pulp, pass through a 40-mesh sieve, and stir well to prepare abalone viscera pretreatment solution, centrifuge, remove sediment, and take Serum. A membrane stack of 40 membrane pairs is used, and the supernatant is subjected to electrodialysis treatment with a voltage of 24V to obtain the light phase. Concentrate the light-phase treatment solution until the soluble solids content reaches 5%, to obtain a concentrated solution, add 95% alcohol according to 2.5 times the volume of the concentrated solution, let it stand for alcohol precipitation for 11 hours, take the supernatant and carry out rectification to recover ethanol, Then concentrate and freeze-dry to obtain 68g of alcohol-soluble antioxidant substances in abalone viscera. After the sample was tested, the heavy metal content was 7.0ppm, which was two-thirds lower than that of the ...
Embodiment 2
[0027] At 30°C, take 10 kg of abalone viscera that has been cleaned and removed of connective tissue, add 30 kg of distilled water, beat the pulp, pass through a 40-mesh sieve, and stir well to prepare abalone viscera pretreatment solution, centrifuge, remove sediment, and take Serum. A membrane stack of 40 membrane pairs is used to conduct electrodialysis treatment with a voltage of 32V to obtain the light phase. Concentrate the light-phase treatment solution until the soluble solids content reaches 7%, to obtain a concentrated solution, add 95% ethanol with a purity of 3 times the volume of the concentrated solution, let it stand for alcohol precipitation for 10 hours, take the supernatant and carry out rectification to recover ethanol, Then concentrate and freeze-dry to obtain 72g of alcohol-soluble antioxidant substances in abalone viscera. The sample was tested, and the heavy metal content was 5.0ppm, which was three quarters lower than that of the comparative example. ...
Embodiment 3
[0029] At 20°C, take 10 kg of abalone viscera that has been cleaned and removed of connective tissue, add 40 kg of distilled water, beat the pulp, pass through a 40-mesh sieve, and stir well to prepare abalone viscera pretreatment solution, centrifuge, remove sediment, and take supernatant. A membrane stack of 40 membrane pairs is used to conduct electrodialysis treatment with a voltage of 40V to obtain the light phase. Concentrate the light-phase treatment solution until the soluble solids content reaches 9%, to obtain a concentrated solution, add alcohol with a purity of more than 95% according to 3.5 times the volume of the concentrated solution, let stand for alcohol precipitation for 9 hours, and take the supernatant for rectification to recover ethanol , and then concentrated and freeze-dried to obtain 66 g of alcohol-soluble antioxidant substances in abalone viscera. The sample was tested, and the heavy metal content was 5.0ppm, which was three quarters lower than that...
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