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Rabbit haemorrhagic disease tissue inactivated vaccine and preparation method thereof

An inactivated vaccine and tissue technology, which can be used in pharmaceutical formulations, inactivation/attenuation, medical preparations containing active ingredients, etc. Antibody production and enhancement of leukocyte phagocytic ability

Inactive Publication Date: 2014-03-26
河南后羿生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the use of vaccines has been quite popular, due to some reasons, there are still many rabbits in our country that have rabbit plague every year, causing certain economic losses to rabbit farms.

Method used

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  • Rabbit haemorrhagic disease tissue inactivated vaccine and preparation method thereof
  • Rabbit haemorrhagic disease tissue inactivated vaccine and preparation method thereof

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Experimental program
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Embodiment 2

[0023] The rabbit plague tissue inactivated vaccine of this embodiment contains rabbit plague tissue virus liquid and propolis adjuvant, and the addition ratio is: 10 mg of propolis is contained in each milliliter of virus extract.

Embodiment 3

[0025] The rabbit plague tissue inactivated vaccine of this embodiment contains rabbit plague tissue virus liquid and propolis adjuvant, and the addition ratio is: 5 mg of propolis is contained in each milliliter of virus extract.

[0026] Preparation method embodiment of rabbit plague tissue inactivated vaccine

[0027] The preparation method of the rabbit plague tissue inactivated vaccine of the present embodiment is as follows:

[0028] 1) Treatment of diseased materials: autopsy dead rabbits with typical symptoms of rabbit plague, aseptically take more than half of the liver and add sterile saline at a ratio of 1:5, plus penicillin and streptomycin 1000U (ug) / ml, and place for 4 Centrifuge at 4000rpm / min for 5 minutes, freeze and thaw 3 times at 10000rpm / min for 5 minutes, take the supernatant and purify it by centrifugation at 10000rpm / min for 30 minutes at 4℃, and store the supernatant aseptically for later use.

[0029] 2) Rabbit subculture: 1ml / mouse of the above-ment...

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Abstract

The invention relates to a rabbit haemorrhagic disease tissue inactivated vaccine and a preparation method thereof. The rabbit haemorrhagic disease tissue inactivated vaccine comprises a rabbit haemorrhagic disease tissue virus liquid and a propolis adjuvant, wherein every liter of virus extracting solution contains 5-15mg of propolis. The preparation method mainly comprises the following steps of: treating pathological materials of a disease and obtaining a virus liquid; inactivating viruses; preparing a vaccine; inspecting the safety and immune efficiency of the vaccine; and performing split charging on the vaccine. A vaccine pathogen is passed and proliferated after separation, and comprehensive antigen components are used for comprehensively inducing inoculated animal groups on multiple aspects, so that good effects of preventing and controlling diseases can be achieved. The adopted propolis adjuvant is a good immunopotentiator as well as an irritant, has broad spectrum biologic activity, can be used for causing specific immune response and starting a nonspecific defense mechanism, and has the remarkable effects of improving immune antibody generation and increasing the leucocyte phagocytosis.

Description

technical field [0001] The invention relates to a tissue inactivated vaccine against rabbit plague, and also relates to a preparation method of the rabbit plague tissue inactivated vaccine, which belongs to the field of veterinary animal vaccines. Background technique [0002] Rabbit viral hemorrhagic disease (RHD), commonly known as "rabbit plague", or rabbit hemorrhagic disease, is an acute, highly contagious infectious disease of rabbits caused by rabbit viral hemorrhagic disease virus (RHDV), characterized by the appearance of dead rabbits Systemic sepsis changes, various organs have different degrees of bleeding, congestion and edema. The lungs were highly edematous, with hemorrhagic spots of various sizes, and a large amount of red foamy liquid flowed out from the cut surface. Throat and tracheal mucous membrane congestion or diffuse hemorrhage, especially the tracheal ring; liver swelling and degeneration, which is earthy yellow, or congestion is purple, with bleedin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/12A61K39/39A61P31/14C12N7/02C12N7/06
Inventor 舒黛莲王卫芳孙芳
Owner 河南后羿生物工程股份有限公司